金鱼视网膜中表达的一种新型烟碱型乙酰胆碱受体结构亚基的鉴定。
Identification of a novel nicotinic acetylcholine receptor structural subunit expressed in goldfish retina.
作者信息
Cauley K, Agranoff B W, Goldman D
机构信息
Mental Health Research Institute.
出版信息
J Cell Biol. 1989 Feb;108(2):637-45. doi: 10.1083/jcb.108.2.637.
Abstract
A new non-alpha (n alpha) member of the nicotinic acetylcholine receptor (nAChR) gene family designated GFn alpha-2 has been identified in goldfish retina by cDNA cloning. This cDNA clone encodes a protein with structural features common to all nAChR subunits sequenced to date; however, unlike all known alpha-subunits of the receptor, it lacks the cysteine residues believed to be involved in acetylcholine binding. Northern blot analysis shows multiple transcripts hybridizing to the GFn alpha-2 cDNA in goldfish retina but undetectable levels of hybridizable RNA in brain, muscle, or liver. S1 nuclease protection experiments indicate that multiple mRNAs are expressed in retina with regions identical or very similar to the GFn alpha-2 sequence. In situ hybridization shows that the gene encoding GFn alpha-2 is expressed predominantly in the ganglion cell layer of the retina.
摘要
通过cDNA克隆在金鱼视网膜中鉴定出烟碱型乙酰胆碱受体（nAChR）基因家族的一个新的非α（nα）成员，命名为GFnα-2。该cDNA克隆编码一种蛋白质，其具有迄今测序的所有nAChR亚基共有的结构特征；然而，与该受体所有已知的α亚基不同，它缺乏据信参与乙酰胆碱结合的半胱氨酸残基。Northern印迹分析显示，在金鱼视网膜中有多个转录本与GFnα-2 cDNA杂交，但在脑、肌肉或肝脏中未检测到可杂交RNA的水平。S1核酸酶保护实验表明，视网膜中表达多种mRNA，其区域与GFnα-2序列相同或非常相似。原位杂交显示，编码GFnα-2的基因主要在视网膜的神经节细胞层中表达。
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