Xia Xinchao, Wang Hui, Niu Xu, Wang Haijun, Liu Zhiqi, Liu Yongming, Qi Zhiming, Wang Shengyi, Liu Shixiang, Liu Shanshan
Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of New Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutics Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, Gansu, China.
Faculté de Médecine, Université de Bretagne Occidentale, 29238 Brest, France 4 Lanzhou Productivity Promoting Center, Lanzhou 730030. China.
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):140-147. doi: 10.4314/ajtcam.v11i1.22. eCollection 2014.
Diarrhea is a big problem in piglets. Cangpo Oral Liquid (COL) is a compound of Chinese herbal medicine. The preparation was fed to piglets had diarrheal disease in order to determine its anti-diarrhea activity and potential applications in vivo.
The contents of Berberine hydrochloride, Magnolol and Honokiol in COL were performed on HPLC analysis. Organ bath was used to investigate the effect of COL on peristaltic reflexes and peristaltic waves in vitro. And anti-diarrhea activity of COL was evaluated in clinical.
Thin layer chromatography (TLC) and HPLC analyses showed that the contents of Berberine hydrochloride, Magnolol and Honokiol in COL were 970µg/mL, 130µg/mL and 300µg/mL, respectively. Administration of the COL to the organ bath caused a concentration-dependent inhibition of intestinal peristalsis. When the COL concentration in the bath was cumulatively increased, the amplitude and frequency of the peristaltic waves was lowered. The result of clinical efficacy of COL was very effective to diarrheic piglets. COL can possibly inhibit the curve of peristaltic waves in vitro; and clinical trial showed a statistically significant therapeutic effect in vivo.
In conclusion, COL can be used as an effective therapeutic agent. However, the ingredients, pharmacokinetics and specific signaling pathways of COL need to be further studied.
腹泻是仔猪面临的一个大问题。苍朴口服液（COL）是一种中药复方制剂。为确定其抗腹泻活性及在体内的潜在应用，将该制剂喂给患有腹泻病的仔猪。
采用高效液相色谱法（HPLC）分析COL中盐酸小檗碱、厚朴酚和和厚朴酚的含量。利用离体器官浴槽研究COL对体外蠕动反射和蠕动波的影响。并对COL的抗腹泻活性进行临床评价。
薄层色谱法（TLC）和HPLC分析表明，COL中盐酸小檗碱、厚朴酚和和厚朴酚的含量分别为970μg/mL、130μg/mL和300μg/mL。向器官浴槽中加入COL可引起肠道蠕动的浓度依赖性抑制。当浴槽中COL浓度累积增加时，蠕动波的幅度和频率降低。COL对腹泻仔猪的临床疗效结果非常有效。COL可能在体外抑制蠕动波曲线；临床试验表明其在体内具有统计学显著的治疗效果。
总之，COL可作为一种有效的治疗药物。然而，COL的成分、药代动力学和具体信号通路需要进一步研究。
