Amang Perfusion A, Tan Paul V, Patamaken Simon A, Mefe Martial N
Department of Animal Biology and Physiology, Faculty of Science, P.O. Box 812, University of Yaounde I, Cameroon.
Afr J Tradit Complement Altern Med. 2013 Nov 2;11(1):165-71. doi: 10.4314/ajtcam.v11i1.26. eCollection 2014.
Ethno-botanical information shows that Eremomastax speciosa is used in the traditional management of various stomach complaints including gastro-duodenal ulcers.
In this study, we tested the cytoprotective potential of the whole plant methanol extract (100-200 mg/kg, p.o), against HCl/ethanol, absolute ethanol, cold/restraint stress rats, and pylorus legated rats pre-treated with indomethacin. The effects of the extract on gastric lesion inhibition, the volume of gastric juice, gastric pH, gastric acid output, mucus production and gastric peptic activity were recorded. Oxidative stress parameters were measured in blood and gastric tissue samples obtained from the animals in all the models tested.
The extract significantly (p<0.05), reduced the formation of cold/restraint ulcers by (31-60%, inhibition), completely inhibited (100%) the formation of lesions induced by HCl/ethanol at the highest dose, but was less effective against absolute ethanol (22-46% inhibition). The extract (200 mg/kg), significantly reduced lesion formation (P<0.01), gastric acidity (P<0.01), and volume of gastric secretions (P<0.05), in the indomethacin/pylorus ligation model, and did not affect the activity of pepsin in gastric juice. Blood concentrations of antioxidant enzymes (catalase, SOD and GSH), increased significantly and MDA concentrations decreased in all models tested.
Cytoprotection by E. speciosa methanol extract was attributed to its ability to reduce acid secretion, and to enhance mucosal defence and in vivo antioxidant status.
民族植物学信息表明，美丽曲柄草被用于传统医学治疗包括胃十二指肠溃疡在内的各种胃部疾病。
在本研究中，我们测试了全株甲醇提取物（100 - 200毫克/千克，口服）对盐酸/乙醇、无水乙醇、冷束缚应激大鼠以及用吲哚美辛预处理的幽门结扎大鼠的细胞保护潜力。记录了提取物对胃损伤抑制、胃液体积、胃pH值、胃酸分泌、黏液产生和胃蛋白酶活性的影响。在所有测试模型的动物血液和胃组织样本中测量了氧化应激参数。
提取物显著（p<0.05）减少了冷束缚溃疡的形成（抑制率为31 - 60%），在最高剂量时完全抑制（100%）了盐酸/乙醇诱导的损伤形成，但对无水乙醇的效果较差（抑制率为22 - 46%）。在吲哚美辛/幽门结扎模型中，提取物（200毫克/千克）显著减少了损伤形成（P<0.01）、胃酸度（P<0.01）和胃液分泌量（P<0.05），并且不影响胃液中胃蛋白酶的活性。在所有测试模型中，抗氧化酶（过氧化氢酶、超氧化物歧化酶和谷胱甘肽）的血液浓度显著升高，丙二醛浓度降低。
美丽曲柄草甲醇提取物的细胞保护作用归因于其减少胃酸分泌、增强黏膜防御和体内抗氧化状态的能力。
