Vandi Vandza Luc, Amang André Perfusion, Mezui Christophe, Siwe Gaël Tchokomeni, Ndji Gustave Lebeau Otto, Mbida Hacheked, Baponwa Odile, Tan Paul Vernyuy
Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 047, Yaoundé, Cameroon.
Evid Based Complement Alternat Med. 2022 Jan 31;2022:5190499. doi: 10.1155/2022/5190499. eCollection 2022.
The objective of this study was to elucidate the antisecretory mechanism of the root bark aqueous extract of (RBAEDM) in Wistar rats. . RBAEDM was tested on three experimental animal models of gastric acid hypersecretion including pyloric ligation (PL), PL with histamine, and carbachol pretreatments. The ulcerated surface, mucus mass, pH, gastric acidity, and pepsin activity were determined. Some bioactive compounds revealed by qualitative phytochemistry were quantified. Some markers of oxidative stress such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and antioxidant tests (ABTS: 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid, DPPH: 2,2-diphenyl-2-picrylhydrazyl, and FRAP: ferric reducing antioxidant power) were determined.
In the three models studied, RBAEDM resulted in increases in the percentages of inhibition ranging from 9.50 to 59.52% of gastric ulcer and mucus mass. This increase was accompanied by the reduction in acidity and pepsin activity. The administration of RBAEDM resulted in a significant decrease ( < 0.05, < 0.01) in MDA levels correlated with a significant increase ( < 0.05, < 0.01) in CAT and nitrite levels compared with the negative control. RBAEDM has the ability to scavenge ABTS and DPPH radicals and to reduce FRAP, and the inhibitory concentration of 50% (IC) of the ABTS radical was 220 g/mL compared with the butylhydroxytoluene (BHT) control (175 g/mL). Quantitative phytochemistry revealed abundant polyphenols, flavonoids, tannins, saponins, and anthocyanin.
RBAEDM protected gastric mucous membrane for gastric acid by mechanisms that would involve both anticholinergic and antihistaminergic pathways.
本研究旨在阐明地锦草根皮水提取物（RBAEDM）对Wistar大鼠的抗分泌机制。RBAEDM在三种胃酸分泌过多的实验动物模型上进行了测试，包括幽门结扎（PL）、组胺预处理的PL以及卡巴胆碱预处理。测定了溃疡表面、黏液量、pH值、胃酸度和胃蛋白酶活性。对定性植物化学分析显示的一些生物活性化合物进行了定量。测定了一些氧化应激标志物，如丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）以及抗氧化试验（ABTS：2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸、DPPH：2,2-二苯基-1-苦基肼、FRAP：铁还原抗氧化能力）。
在研究的三种模型中，RBAEDM使胃溃疡和黏液量的抑制率提高了9.50%至59.52%。这种增加伴随着酸度和胃蛋白酶活性的降低。与阴性对照相比，给予RBAEDM导致MDA水平显著降低（P<0.05，P<0.01），同时CAT和亚硝酸盐水平显著升高（P<0.05，P<0.01）。RBAEDM具有清除ABTS和DPPH自由基以及降低FRAP的能力，与丁基羟基甲苯（BHT）对照（175μg/mL）相比，ABTS自由基的50%抑制浓度（IC）为220μg/mL。定量植物化学分析显示含有丰富的多酚、黄酮类、单宁、皂苷和花青素。
RBAEDM通过涉及抗胆碱能和抗组胺能途径的机制保护胃黏膜免受胃酸侵害。
