溶液核磁共振中的灵敏度增强：新观点与新前沿
Sensitivity enhancement in solution NMR: emerging ideas and new frontiers.
作者信息
Lee Jung Ho, Okuno Yusuke, Cavagnero Silvia
机构信息
Department of Chemistry and Biophysics Program, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
Department of Chemistry and Biophysics Program, University of Wisconsin-Madison, 1101 University Avenue, Madison, WI 53706-1322, USA.
出版信息
J Magn Reson. 2014 Apr;241:18-31. doi: 10.1016/j.jmr.2014.01.005.
Abstract
Modern NMR spectroscopy has reached an unprecedented level of sophistication in the determination of biomolecular structure and dynamics at atomic resolution in liquids. However, the sensitivity of this technique is still too low to solve a variety of cutting-edge biological problems in solution, especially those that involve viscous samples, very large biomolecules or aggregation-prone systems that need to be kept at low concentration. Despite the challenges, a variety of efforts have been carried out over the years to increase sensitivity of NMR spectroscopy in liquids. This review discusses basic concepts, recent developments and future opportunities in this exciting area of research.
摘要
现代核磁共振光谱学在测定液体中生物分子的原子分辨率结构和动力学方面已达到了前所未有的复杂程度。然而，该技术的灵敏度仍然过低，无法解决溶液中的各种前沿生物学问题，尤其是那些涉及粘性样品、非常大的生物分子或需要保持低浓度的易聚集体系的问题。尽管存在挑战，但多年来人们已经做出了各种努力来提高液体核磁共振光谱学的灵敏度。本综述讨论了这一令人兴奋的研究领域的基本概念、最新进展和未来机遇。
相似文献
1
Sensitivity enhancement in solution NMR: emerging ideas and new frontiers.
J Magn Reson. 2014 Apr;241:18-31. doi: 10.1016/j.jmr.2014.01.005.
2
A novel tri-enzyme system in combination with laser-driven NMR enables efficient nuclear polarization of biomolecules in solution.
J Phys Chem B. 2013 May 23;117(20):6069-81. doi: 10.1021/jp4010168. Epub 2013 Apr 30.
3
Laser- and cryogenic probe-assisted NMR enables hypersensitive analysis of biomolecules at submicromolar concentration.
Proc Natl Acad Sci U S A. 2019 Jun 11;116(24):11602-11611. doi: 10.1073/pnas.1820573116. Epub 2019 May 29.
4
1H-Detected 13C photo-CIDNP as a sensitivity enhancement tool in solution NMR.
J Am Chem Soc. 2011 Jun 1;133(21):8062-5. doi: 10.1021/ja111613c. Epub 2011 May 6.
5
Structure determination of biological macromolecules in solution using nuclear magnetic resonance spectroscopy.
Biotechniques. 2000 Dec;29(6):1278-82, 1284-90, 1292 passim. doi: 10.2144/00296ra01.
6
Photo-CIDNP MAS NMR in intact cells of Rhodobacter sphaeroides R26: molecular and atomic resolution at nanomolar concentration.
J Am Chem Soc. 2006 Oct 4;128(39):12794-9. doi: 10.1021/ja0623616.
7
Improved sensitivity of laser-enhanced H-C-correlation via suppression of C-C' scalar-coupling evolution.
J Magn Reson. 2019 Oct;307:106572. doi: 10.1016/j.jmr.2019.106572. Epub 2019 Aug 13.
8
Structure determination using solution NMR: Is it worth the effort?
J Magn Reson. 2019 Sep;306:195-201. doi: 10.1016/j.jmr.2019.07.045. Epub 2019 Jul 13.
9
Recent advances in solution NMR: fast methods and heteronuclear direct detection.
Chemphyschem. 2009 Jul 13;10(9-10):1356-68. doi: 10.1002/cphc.200900133.
10
Rapid sample-mixing technique for transient NMR and photo-CIDNP spectroscopy: applications to real-time protein folding.
J Am Chem Soc. 2003 Oct 15;125(41):12484-92. doi: 10.1021/ja036357v.
引用本文的文献
1
Overhauser Dynamic Nuclear Polarization Enables Single Scan Benchtop C NMR Spectroscopy in Continuous-Flow.
Anal Chem. 2025 Mar 4;97(8):4308-4317. doi: 10.1021/acs.analchem.4c03985. Epub 2025 Feb 21.
2
Harnessing NMR technology for enhancing field crop improvement: applications, challenges, and future perspectives.
Metabolomics. 2025 Feb 20;21(2):27. doi: 10.1007/s11306-025-02229-z.
3
RadicalPy: A Tool for Spin Dynamics Simulations.
J Chem Theory Comput. 2024 Nov 12;20(21):9488-9499. doi: 10.1021/acs.jctc.4c00887. Epub 2024 Oct 29.
4
Hyperpolarized C NMR Reveals Pathway Regulation in and Metabolic Similarities and Differences Across the Tree of Life.
Molecules. 2024 Aug 30;29(17):4133. doi: 10.3390/molecules29174133.
5
Large-enhancement nanoscale dynamic nuclear polarization near a silicon nanowire surface.
Sci Adv. 2024 Aug 23;10(34):eado9059. doi: 10.1126/sciadv.ado9059. Epub 2024 Aug 21.
6
Possibilities and limitations of solution-state NMR spectroscopy to analyze the ligand shell of ultrasmall metal nanoparticles.
Nanoscale Adv. 2024 May 31;6(13):3285-3298. doi: 10.1039/d4na00139g. eCollection 2024 Jun 25.
7
The Complementarity of Nuclear Magnetic Resonance and Native Mass Spectrometry in Probing Protein-Protein Interactions.
Adv Exp Med Biol. 2024;3234:109-123. doi: 10.1007/978-3-031-52193-5_8.
8
LC-Photo-CIDNP hyperpolarization of biomolecules bearing a quasi-isolated spin pair: Magnetic-Field dependence via a rapid-shuttling device.
J Magn Reson. 2024 Feb;359:107616. doi: 10.1016/j.jmr.2023.107616. Epub 2023 Dec 29.
9
Detection of sub-nmol amounts of the antiviral drug favipiravir in F MRI using photo-chemically induced dynamic nuclear polarization.
Sci Rep. 2024 Jan 17;14(1):1527. doi: 10.1038/s41598-024-51454-4.
10
Hyperpolarization-Enhanced NMR Spectroscopy of Unaltered Biofluids Using Photo-CIDNP.
Anal Chem. 2024 Jan 9;96(1):102-109. doi: 10.1021/acs.analchem.3c03215. Epub 2023 Dec 18.
本文引用的文献
1
Creating Long-Lived Spin States at Variable Magnetic Field by Means of Photochemically Induced Dynamic Nuclear Polarization.
J Phys Chem Lett. 2012 Jul 5;3(13):1814-9. doi: 10.1021/jz3005046. Epub 2012 Jun 22.
2
Boosting Dissolution Dynamic Nuclear Polarization by Cross Polarization.
J Phys Chem Lett. 2013 Jan 3;4(1):111-4. doi: 10.1021/jz301781t. Epub 2012 Dec 18.
3
Hydration dynamics as an intrinsic ruler for refining protein structure at lipid membrane interfaces.
Proc Natl Acad Sci U S A. 2013 Oct 15;110(42):16838-43. doi: 10.1073/pnas.1307678110. Epub 2013 Sep 30.
4
Near-unity nuclear polarization with an open-source 129Xe hyperpolarizer for NMR and MRI.
Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):14150-5. doi: 10.1073/pnas.1306586110. Epub 2013 Aug 14.
5
Protein folding studied by dissolution dynamic nuclear polarization.
Angew Chem Int Ed Engl. 2013 Aug 26;52(35):9192-5. doi: 10.1002/anie.201301851. Epub 2013 Jul 15.
6
Hyperpolarized Hadamard spectroscopy using flow NMR.
Anal Chem. 2013 Aug 6;85(15):7385-90. doi: 10.1021/ac401293n. Epub 2013 Jul 8.
7
Nanoemulsion contrast agents with sub-picomolar sensitivity for xenon NMR.
J Am Chem Soc. 2013 Jul 3;135(26):9576-9. doi: 10.1021/ja402885q. Epub 2013 Jun 18.
8
Photo-CIDNP NMR spectroscopy of amino acids and proteins.
Top Curr Chem. 2013;338:229-300. doi: 10.1007/128_2013_427.
9
A novel tri-enzyme system in combination with laser-driven NMR enables efficient nuclear polarization of biomolecules in solution.
J Phys Chem B. 2013 May 23;117(20):6069-81. doi: 10.1021/jp4010168. Epub 2013 Apr 30.
10
Liquid state DNP of water at 9.2 T: an experimental access to saturation.
Phys Chem Chem Phys. 2013 Apr 28;15(16):6049-56. doi: 10.1039/c3cp44461a. Epub 2013 Mar 15.
Zotero 插件