薯蓣皂苷元通过抑制 Vav2 活性抑制人乳腺癌 MDA-MB-231 细胞的迁移。
Diosgenin inhibits the migration of human breast cancer MDA-MB-231 cells by suppressing Vav2 activity.
机构信息
College of Chinese Medicine Materials, Jilin Agricultural University, Changchun 130118, China.
The First Affiliated Hospital of Changchun University of Traditional Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130021, China.
出版信息
Phytomedicine. 2014 May 15;21(6):871-6. doi: 10.1016/j.phymed.2014.02.002. Epub 2014 Mar 18.
Diosgenin, a naturally occurring steroidal saponin, possess tumor therapeutic potential. However, the effect of diosgenin on cancer metastasis remains poorly understood. In this study, we performed in vitro experiments to investigate the inhibitory activity of diosgenin on human breast cancer MDA-MB-231 cell migration, and reveal the possible mechanism. Diosgenin caused a marked inhibition of cell migration in MDA-MB-231 cell by transwell assay. In addition, diosgenin significantly impacted MDA-MB-231 cell migratory behavior under real-time observation. We also found diosgenin significantly inhibited actin polymerization, Vav2 phosphorylation and Cdc42 activation, which might be, at least in part, attributed to the anti-metastatic potential of diosgenin. These findings reveal a new therapeutic potential of diosgenin for human breast cancer metastasis therapy.
薯蓣皂苷元是一种天然甾体皂苷，具有肿瘤治疗潜力。然而，薯蓣皂苷元对癌症转移的影响仍知之甚少。在这项研究中，我们进行了体外实验，以研究薯蓣皂苷元对人乳腺癌 MDA-MB-231 细胞迁移的抑制活性，并揭示可能的机制。通过 Transwell 分析，薯蓣皂苷元明显抑制 MDA-MB-231 细胞迁移。此外，在实时观察下，薯蓣皂苷元显著影响 MDA-MB-231 细胞的迁移行为。我们还发现薯蓣皂苷元显著抑制肌动蛋白聚合、Vav2 磷酸化和 Cdc42 激活，这至少部分归因于薯蓣皂苷元的抗转移潜力。这些发现揭示了薯蓣皂苷元在人类乳腺癌转移治疗中的新治疗潜力。
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