Zhang Yandong, Wu Wei, Kang Lihua, Yu Dehai, Liu Chunshui
Department of Rheumatology, The First Hospital of Jilin University, Changchun, Jilin Province, 130021, China.
Department of Neurosurgery, The First Hospital of Jilin University, Changchun, Jilin Province, 130021, China.
Int J Biol Macromol. 2017 Oct;103:477-483. doi: 10.1016/j.ijbiomac.2017.05.084. Epub 2017 May 17.
Polysaccharides extracted from medicinal plants possess multiple functions. However, the inhibitory capacity of polysaccharides on the metastasis of breast cancer remains unclear. In the present study, we investigated the inhibitory activity of Aconitum coreanum polysaccharide (ACP1) and its sulphated derivative ACP1-s on migratory behaviour of human breast cancer cells MDA-MB-435s and evaluated the underlying molecular mechanism. The data from Transwell assay indicated that ACP1 and ACP1-s caused a significant inhibition of MDA-MB-435s cell migration in vitro. ACP1 and ACP1-s significantly impaired MDA-MB-435s cell migratory behaviour, and the accumulated distance and average velocity of ACP1- and ACP1-s-treated cells were reduced markedly. We also found ACP1 and ACP1-s treatment could affect dynamic remodeling of actin cytoskeleton, and suppress phosphorylation and activation of signalling molecules, attributing to anti-metastatic role of ACP1 and ACP1-s. These findings reveal a novel therapeutic potential of A. coreanum polysaccharide and its sulphated derivative for breast cancer metastasis.
从药用植物中提取的多糖具有多种功能。然而，多糖对乳腺癌转移的抑制能力仍不清楚。在本研究中，我们研究了朝鲜乌头多糖（ACP1）及其硫酸化衍生物ACP1-s对人乳腺癌细胞MDA-MB-435s迁移行为的抑制活性，并评估了其潜在的分子机制。Transwell实验数据表明，ACP1和ACP1-s在体外对MDA-MB-435s细胞迁移具有显著抑制作用。ACP1和ACP1-s显著损害了MDA-MB-435s细胞的迁移行为，经ACP1和ACP1-s处理的细胞的累积距离和平均速度明显降低。我们还发现，ACP1和ACP1-s处理可影响肌动蛋白细胞骨架的动态重塑，并抑制信号分子的磷酸化和激活，这归因于ACP1和ACP1-s的抗转移作用。这些发现揭示了朝鲜乌头多糖及其硫酸化衍生物在治疗乳腺癌转移方面的新潜力。
