Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India.
Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007, India.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:20-7. doi: 10.1016/j.msec.2014.01.038. Epub 2014 Jan 29.
We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ~3 nm and showed bright blue fluorescence under UV-light (λex=365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae).
我们报道了一种高效经济的植物基水热法，通过使用甘蔗汁作为前体制备可分散在水中的荧光碳点（CDs）。合成的 CDs 通过紫外-可见分光光度法、荧光分光光度法、傅里叶变换红外光谱（FT-IR）、动态光散射（DLS）、高分辨率透射电子显微镜（HR-TEM）和激光扫描共聚焦显微镜技术进行了表征。这些 CDs 在水中具有良好的分散性，平均尺寸约为 3nm，并在紫外光（λex=365nm）下显示出明亮的蓝色荧光。这些 CDs 可作为细菌（大肠杆菌）和酵母（酿酒酵母）细胞成像的优良荧光探针。
