利用甘蔗汁一锅法绿色合成碳点用于细菌（大肠杆菌）和酵母（酿酒酵母）细胞的荧光成像。
One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells.
机构信息
Applied Chemistry Department, S. V. National Institute of Technology, Surat, 395 007, India.
Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007, India.
出版信息
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:20-7. doi: 10.1016/j.msec.2014.01.038. Epub 2014 Jan 29.
We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ~3 nm and showed bright blue fluorescence under UV-light (λex=365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae).
我们报道了一种高效经济的植物基水热法，通过使用甘蔗汁作为前体制备可分散在水中的荧光碳点（CDs）。合成的 CDs 通过紫外-可见分光光度法、荧光分光光度法、傅里叶变换红外光谱（FT-IR）、动态光散射（DLS）、高分辨率透射电子显微镜（HR-TEM）和激光扫描共聚焦显微镜技术进行了表征。这些 CDs 在水中具有良好的分散性，平均尺寸约为 3nm，并在紫外光（λex=365nm）下显示出明亮的蓝色荧光。这些 CDs 可作为细菌（大肠杆菌）和酵母（酿酒酵母）细胞成像的优良荧光探针。
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