Department of Biochemistry and Molecular Biology, University of Bucharest, Spl. Independentei, 91-95, 050095 Bucharest, Romania.
Department of Electrochemistry and Corrosion, Institute of Physical Chemistry "Ilie Murgulescu" of Romanian Academy, Spl. Independentei 202, 060021 Bucharest, Romania.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:127-42. doi: 10.1016/j.msec.2014.01.056. Epub 2014 Feb 7.
A new Ti-25Ta-5Zr alloy based only on non-toxic and non-allergic elements was elaborated in as-cast and thermo-mechanical processed, recrystallized states (XRD and SEM) in order to be used as candidate material for implant applications. Its long-term interactions with Ringer-Brown and Ringer solutions of different pH values and its cytocompatibility were determined. The thermo-mechanically processed alloy has nobler electrochemical behaviour than as-cast alloy due to finer microstructure obtained after the applied treatment. Corrosion and ion release rates presented the lowest values for the treated alloy. Nyquist and Bode plots displayed higher impedance values and phase angles for the processed alloy, denoting a more protective passive film. SEM micrographs revealed depositions from solutions that contain calcium, phosphorous and oxygen ions (EDX analysis), namely calcium phosphate. An electric equivalent circuit with two time constants was modelled. Cell culture experiments with MC3T3-E1 pre-osteoblasts demonstrated that thermo-mechanically processed Ti-25Ta-5Zr alloy supports a better cell adhesion and spreading, and enhanced cell proliferation. Altogether, these data indicate that thermo-mechanical treatment endows the alloy with improved anticorrosion and biological performances.
研发了一种新的 Ti-25Ta-5Zr 合金，它仅基于无毒且无致敏性的元素，以铸态和热机械加工、再结晶态（XRD 和 SEM）进行了研究，旨在作为植入物应用的候选材料。研究了其与不同 pH 值的林格-布朗和林格溶液的长期相互作用及其细胞相容性。由于经过处理后获得了更细的微观结构，因此热机械加工的合金具有比铸态合金更优异的电化学性能。处理后的合金具有最低的腐蚀和离子释放速率。奈奎斯特和波特图显示处理后的合金具有更高的阻抗值和相位角，表明其具有更具保护性的钝化膜。SEM 显微照片显示了从含有钙、磷和氧离子的溶液中（EDX 分析）沉积的物质，即磷酸钙。建立了一个具有两个时间常数的等效电路模型。用 MC3T3-E1 前成骨细胞进行细胞培养实验表明，热机械加工的 Ti-25Ta-5Zr 合金具有更好的细胞黏附和铺展能力，以及增强的细胞增殖能力。综上所述，这些数据表明，热机械处理赋予了合金更好的耐腐蚀性和生物学性能。
