University of Bucharest, Department of Biochemistry and Molecular Biology, 91-95 Spl. Independentei, 050095, Bucharest, Romania.
INSA Rennes, UMR CNRS 6226 SCR/Chimie-Métallurgie, 20 avenue des Buttes de Coësmes, F-35043 Rennes Cedex, France.
Mater Sci Eng C Mater Biol Appl. 2012 Aug 1;32(6):1554-63. doi: 10.1016/j.msec.2012.04.042. Epub 2012 Apr 29.
Among metallic materials used as bone substitutes, β titanium alloys gain an increasing importance because of their low modulus, high corrosion resistance and good biocompatibility. In this work, an investigation of the in vitro cytocompatibility of a recently new developed β-type Ti-25Ta-25Nb alloy was carried out by evaluating the behavior of human osteoblasts. The metallic Ti-6Al-4V biomaterial, which is one of representative α+β type titanium alloys for biomedical applications, and Tissue Culture Polystyrene (TCPS), were also investigated as reference Ti-based material and control substrate, respectively. Both metallic surfaces were analyzed by X-ray diffraction, atomic force microscopy and X-ray photoelectron spectroscopy. The cellular response was quantified by assessments of viability, cell attachment and spreading, cell morphology, production and extracellular organization of fibronectin and cell proliferation. Polished surfaces from both materials having an equiaxed grain microstructure and nanometre scale surface roughness elicited an essentially identical osteoblast response in terms of all analyzed cellular parameters. Thus, on both surfaces the cells displayed high survival rates, good cell adhesion and spreading, a dense and randomly dispersed fibronectin matrix and increasing cell proliferation rates over the incubation time. Furhermore, the enhanced biological performance of Ti-25Ta-25Nb was highly supported by the results obtained in comparison with TCPS. These findings, together with previously shown superelastic behavior, low Young's modulus and high corrosion resistance, recommend Ti-25Ta-25Nb as good candidate for applications in bone implantology.
在用作骨替代物的金属材料中，β 钛合金因其低模量、高耐腐蚀性和良好的生物相容性而越来越受到重视。在这项工作中，通过评估人成骨细胞的行为，研究了最近开发的新型β型 Ti-25Ta-25Nb 合金的体外细胞相容性。生物医学应用中具有代表性的α+β 型钛合金之一的医用 Ti-6Al-4V 生物材料和组织培养聚苯乙烯（TCPS）也分别作为参考 Ti 基材料和对照基底进行了研究。通过 X 射线衍射、原子力显微镜和 X 射线光电子能谱对金属表面进行了分析。通过评估细胞活力、细胞附着和铺展、细胞形态、纤维连接蛋白的产生和细胞外组织以及细胞增殖，对细胞反应进行了量化。两种材料的抛光表面均具有等轴晶粒微观结构和纳米级表面粗糙度，在所有分析的细胞参数方面都引起了基本相同的成骨细胞反应。因此，在两种表面上，细胞均显示出高存活率、良好的细胞附着和铺展、密集且随机分散的纤维连接蛋白基质以及随着孵育时间的延长而增加的细胞增殖率。此外，与 TCPS 相比，Ti-25Ta-25Nb 的增强生物学性能得到了高度支持。这些发现，加上之前显示的超弹性行为、低杨氏模量和高耐腐蚀性，推荐 Ti-25Ta-25Nb 作为骨植入物应用的良好候选材料。
