Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China.
Shanghai Institute of Ceramics, Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, Shanghai 200050, PR China.
Mater Sci Eng C Mater Biol Appl. 2014 May 1;38:272-7. doi: 10.1016/j.msec.2014.02.004. Epub 2014 Feb 13.
In this work, nano/micro hierarchical hybrid structured surface was prepared by fabricating a titania nanotube layer in plasma sprayed porous titanium coating (TC). In vitro human marrow stem cells (hMSCs) were employed for the evaluation of the biological properties of the anodized titanium coating with a hierarchical structure (HSTC). Significantly higher cell adhesion quantity (about 30% more) was found on the HSTC than that on the as-sprayed TC. The expressions of osteocalcin (OC) and osteopontin (OPN) for the HSTC were also detected to be about twice as high as those on the as-sprayed TC. The enhanced cell responses on the HSTC were explained by the improved protein adhesion resulted from the increased surface area and surface energy. Combining the advantages in the mechanical fixation and long-term stability of the plasma sprayed porous TC, the HSTC with a hierarchical structure may be a good candidate for hard tissue replacements, especially for load-bearing implants.
本工作通过在等离子喷涂多孔钛涂层（TC）上制备二氧化钛纳米管层，制备了纳米/微分级混合结构表面。体外人骨髓间充质干细胞（hMSCs）用于评估具有分级结构（HSTC）的阳极氧化钛涂层的生物学特性。与喷射 TC 相比，在 HSTC 上发现细胞黏附数量明显增加（约增加 30%）。还检测到 HSTC 上的骨钙素（OC）和骨桥蛋白（OPN）的表达也比喷射 TC 高约两倍。HSTC 上增强的细胞反应可归因于蛋白质黏附的改善，这是由于表面积和表面能的增加所致。将等离子喷涂多孔 TC 在机械固定和长期稳定性方面的优势相结合，具有分级结构的 HSTC 可能是硬组织替代物的良好候选物，特别是对于承重植入物。
