一项病例对照研究，评估逆转录聚合酶链反应/电喷雾电离质谱技术与直接荧光抗体法和xTAG呼吸道病毒面板聚合酶链反应的比较。
A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR.
作者信息
Hardick Justin, Sadiq Sufyan, Perelstein Elizabeth, Peterson Stephen, Rothman Richard, Gaydos Charlotte A
机构信息
Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA.
Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
出版信息
Diagn Microbiol Infect Dis. 2014 Jun;79(2):187-9. doi: 10.1016/j.diagmicrobio.2014.02.009. Epub 2014 Feb 24.
Abstract
Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.
摘要
将244份鼻咽拭子样本，采用逆转录聚合酶链反应/电喷雾电离质谱PLEX-ID广谱呼吸道病毒监测试剂盒2.5版进行评估，并与直接荧光抗体法和xTAG呼吸道病毒检测板比较，以确定一致性百分比、敏感性和特异性。敏感性和特异性分别为91%（111/122）和95.1%（116/122）。除副流感病毒外，其他病毒的敏感性为92.9%-100%，特异性为99%-100%。
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J Virol Methods. 2013 Oct;193(1):173-6. doi: 10.1016/j.jviromet.2013.06.006. Epub 2013 Jun 10.
2
Current status and future needs in diagnostics and vaccines for high pathogenicity avian influenza.高致病性禽流感诊断与疫苗的现状及未来需求
Dev Biol (Basel). 2013;135:79-94. doi: 10.1159/000325276. Epub 2013 May 14.
3
Clinical accuracy of a PLEX-ID flu device for simultaneous detection and identification of influenza viruses A and B.PLEX-ID 流感检测仪同时检测和鉴定甲型和乙型流感病毒的临床准确性。
J Clin Microbiol. 2013 Jan;51(1):40-5. doi: 10.1128/JCM.01978-12. Epub 2012 Oct 17.
4
Diagnostic performance of two highly multiplexed respiratory virus assays in a pediatric cohort.两种高通量呼吸道病毒检测方法在儿科患者中的诊断性能比较。
J Clin Virol. 2012 Oct;55(2):168-72. doi: 10.1016/j.jcv.2012.06.019. Epub 2012 Jul 24.
5
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J Clin Virol. 2012 Aug;54(4):332-6. doi: 10.1016/j.jcv.2012.05.002. Epub 2012 Jun 4.
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