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一项病例对照研究,评估逆转录聚合酶链反应/电喷雾电离质谱技术与直接荧光抗体法和xTAG呼吸道病毒面板聚合酶链反应的比较。

A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR.

作者信息

Hardick Justin, Sadiq Sufyan, Perelstein Elizabeth, Peterson Stephen, Rothman Richard, Gaydos Charlotte A

机构信息

Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA.

Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.

出版信息

Diagn Microbiol Infect Dis. 2014 Jun;79(2):187-9. doi: 10.1016/j.diagmicrobio.2014.02.009. Epub 2014 Feb 24.

DOI:10.1016/j.diagmicrobio.2014.02.009
PMID:24657170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4557781/
Abstract

Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.

摘要

将244份鼻咽拭子样本,采用逆转录聚合酶链反应/电喷雾电离质谱PLEX-ID广谱呼吸道病毒监测试剂盒2.5版进行评估,并与直接荧光抗体法和xTAG呼吸道病毒检测板比较,以确定一致性百分比、敏感性和特异性。敏感性和特异性分别为91%(111/122)和95.1%(116/122)。除副流感病毒外,其他病毒的敏感性为92.9%-100%,特异性为99%-100%。

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