Casas-Godoy Leticia, Meunchan Muchalin, Cot Marlène, Duquesne Sophie, Bordes Florence, Marty Alain
Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France.
Université de Toulouse, INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; CNRS, UMR5504, F-31400 Toulouse, France; Department of Biochemistry, Faculty of Science, Khon Kaen University, 123 Mittapap Road, Khon Kaen 40002, Thailand.
J Biotechnol. 2014 Jun 20;180:30-6. doi: 10.1016/j.jbiotec.2014.03.018. Epub 2014 Mar 18.
The production of Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) rich in cis-4,7,10,13,16,19-docosahexaenoic acid (DHA) was studied using lipase-catalysed hydrolysis of a mixture of ethyl esters from tuna oil. Lipases from Yarrowia lipolytica (YLL2), Thermomyces lanuginosus (TLL) and Candida rugosa (CRL1, CRL3 and CRL4) were tested. C. rugosa lipases discriminated esters on the basis of their chain length, with less affinity for γ-linolenate, 11-eicosenoate, arachidonate, EPA, DPA and DHA ethyl esters. However, YLL2 and TLL improved discrimination towards DHA, as enzyme selectivity was shown to be mainly based on the position of the double bond closest to the carboxylic group. From the point of view of kinetics, purity and yield, YLL2 was the most effective lipase for DHA purification. Using this enzyme in an open reactor process resulted in the highest concentrations of DHA ethyl ester (77%) and ω-3 esters (81%) with a recovery of 94% and 77% respectively.
利用脂肪酶催化金枪鱼油乙酯混合物水解，研究了富含顺式-4,7,10,13,16,19-二十二碳六烯酸（DHA）的ω-3多不饱和脂肪酸（PUFA）的生产。测试了解脂耶氏酵母（YLL2）、嗜热栖热菌（TLL）和皱褶假丝酵母（CRL1、CRL3和CRL4）的脂肪酶。皱褶假丝酵母脂肪酶根据酯的链长进行区分，对γ-亚麻酸酯、11-二十碳烯酸酯、花生四烯酸酯、二十碳五烯酸（EPA）、二十二碳五烯酸（DPA）和DHA乙酯的亲和力较低。然而，YLL2和TLL提高了对DHA的区分能力，因为酶的选择性主要基于最靠近羧基的双键位置。从动力学、纯度和产率的角度来看，YLL2是用于DHA纯化的最有效脂肪酶。在开放式反应器工艺中使用这种酶，可得到最高浓度的DHA乙酯（77%）和ω-3酯（81%），回收率分别为94%和77%。
