Boligon Aline A, Piana Mariana, Schawnz Thiago G, Pereira Romaiana P, Rocha João B T, Athayde Margareth L
Nat Prod Commun. 2014 Jan;9(1):61-4.
In this study we evaluated the composition of the crude extract and fractions of Tabernaemontana catharinensis (Apocynaceae) by HPLC/DAD and GC/MS. We also tested the antioxidant capacity and investigated the contents of polyphenols, flavonoids, tannins and alkaloids of T. catharinensis stem bark. The extract and fractions showed inhibition against thiobarbituric acid reactive species (TBARS), in the following order: ethyl acetate (IC50 = 4.7 +/- 0.2 microg/mL) > dichloromethane (23.9 +/- 1.1 microg/mL) > n-butanolic (25.2 +/- 0.4 microg/mL) > crude extract (38.0 +/- 0.07 microg/mL). Moreover, the DPPH assay, presented IC50 values ranged from 5.6 +/- 0.6 to 30.3 +/- 1.3 microg/mL. Contents of total phenols, flavonoids, tannins and alkaloids of T. catharinensis followed the order: ethyl acetate > n-butanolic > dichloromethane fractions > crude extract. HPLC/DAD analyses indicated that gallic, chlorogenic and caffeic acids, and rutin, quercetin and kaempferol are components of the species. Taken together, the results suggest that T. catharinensis could be considered an effective agent in the prevention of diseases associated with oxidative stress.
在本研究中，我们通过高效液相色谱/二极管阵列检测法（HPLC/DAD）和气相色谱/质谱联用仪（GC/MS）对 Catharinensis狗牙花（夹竹桃科）的粗提取物及其馏分的成分进行了评估。我们还测试了其抗氧化能力，并研究了 Catharinensis狗牙花茎皮中多酚、黄酮类化合物、单宁和生物碱的含量。提取物及其馏分对硫代巴比妥酸反应性物质（TBARS）具有抑制作用，顺序如下：乙酸乙酯（IC50 = 4.7 +/- 0.2微克/毫升）>二氯甲烷（23.9 +/- 1.1微克/毫升）>正丁醇（25.2 +/- 0.4微克/毫升）>粗提取物（38.0 +/- 0.07微克/毫升）。此外，1,1-二苯基-2-三硝基苯肼（DPPH）测定法显示IC50值范围为5.6 +/- 0.6至30.3 +/- 1.3微克/毫升。Catharinensis狗牙花中总酚、黄酮类化合物、单宁和生物碱的含量顺序如下：乙酸乙酯>正丁醇>二氯甲烷馏分>粗提取物。HPLC/DAD分析表明，没食子酸、绿原酸和咖啡酸以及芦丁、槲皮素和山奈酚是该物种的成分。综上所述，结果表明 Catharinensis狗牙花可被视为预防与氧化应激相关疾病的有效药物。
