Pavlović R D, Lakusić B, Doslov-Kokorus Z, Kovacević N
Department of Pharmacy, Faculty of Medicine, University of Nis, Nis, Serbia.
Pharmazie. 2009 Oct;64(10):656-9.
Quantitative analyses and investigation of antioxidant activity of herb and dry ethanolic extracts of five species from Ericaceae family (Arbutus unedo L., Bruckentalia spiculifolia Rchb., Calluna vulgaris Salisb., Erica arborea L. and Erica carnea L.) were performed. Total polyphenols, tannins and flavonoids were determined spectrophotometrically and arbutin content was measured both spectrophotometrically and by HPLC coupled with DAD detection. Antioxidative properites of the ethanolic extracts were tested by means of FRAP (total antioxidant capacity), lipid peroxidation and DPPH free radical scavenging activity. A significant amount of arbutin was detected only in Arbutus unedo. All samples investigated showed excellent antioxidant activity. The best inhibition of lipid peroxidation has been shown by Bruckentalia spiculifolia herb extract (62.5 microg/ml; more than 95%), which contained the highest amount of flavonoids (11.79%). The highest scavenging activity was obtained with leave extract of Arbutus unedo (IC50 = 7.14 microg/ml). The leaves of A. unedo contained a small amount of flavonoids but high content of non-tannins polyphenols.
对杜鹃花科五个物种（草莓树、刺叶越桔、帚石楠、树状欧石南和肉色欧石南）的草药及干燥乙醇提取物进行了抗氧化活性的定量分析和研究。采用分光光度法测定总多酚、单宁和黄酮类化合物的含量，并通过分光光度法和高效液相色谱-二极管阵列检测法测定熊果苷含量。通过铁离子还原抗氧化能力（总抗氧化能力）、脂质过氧化和二苯基苦味酰基自由基清除活性测试乙醇提取物的抗氧化性能。仅在草莓树中检测到大量熊果苷。所有研究样品均表现出优异的抗氧化活性。刺叶越桔草药提取物对脂质过氧化的抑制效果最佳（62.5微克/毫升；超过95%），其黄酮类化合物含量最高（11.79%）。草莓树叶片提取物的清除活性最高（半数抑制浓度=7.14微克/毫升）。草莓树的叶子含有少量黄酮类化合物，但非单宁多酚含量较高。
