El-Naggar Medhat A, Thabit Tamer M
1 Plant Pathology Research Institute, Agriculture Research Center , Giza, Egypt .
Foodborne Pathog Dis. 2014 Jun;11(6):433-8. doi: 10.1089/fpd.2013.1711. Epub 2014 Mar 24.
The walls of yeast cells, which contain β-D-glucan biopolymers, have an active role in reducing mycotoxins in animal feed. This study aimed to evaluate the β-D-glucan biopolymers as a mycotoxin binder for fumonisin (FUM) and deoxynivalenol (DON) toxins as well as their effect on the nutritional value of soybean, which is considered one of the important feed row materials. The evaluation was carried out using some toxigenic Fusarium isolates (Fusarium solani, F. oxysporum, and F. verticillioides) in vitro and in vivo. The FUM and DON levels were determined by immune affinity column. The F. verticillioides was the most toxigenic, followed by F. oxysporum and lastly F. solani, while secretion of DON toxin was determined to be greater than FUM with all the tested fungi. The effectiveness of β-D-glucan biopolymers on FUM and DON absorption was greater than clay and calcium propionate. In vivo, treating soybean seeds with β-D-glucan biopolymers led to reduction in the level of FUM and DON toxins in seeds artificially inoculated by F. verticillioides. β-D-glucan treatment also has a low effect on nutritional components of the seeds compared to untreated ones. In conclusion, this study found a new approach to reduce Fusarium mycotoxins in feed to an allowable safe limit and at the same time maintaining the nutritional value of these materials.
酵母细胞壁含有β-D-葡聚糖生物聚合物，在降低动物饲料中的霉菌毒素方面具有积极作用。本研究旨在评估β-D-葡聚糖生物聚合物作为伏马菌素（FUM）和脱氧雪腐镰刀菌烯醇（DON）毒素的霉菌毒素结合剂，以及它们对大豆营养价值的影响，大豆被认为是重要的饲料原料之一。评估是在体外和体内使用一些产毒镰刀菌分离株（茄病镰刀菌、尖孢镰刀菌和轮枝镰刀菌）进行的。FUM和DON水平通过免疫亲和柱测定。轮枝镰刀菌的产毒能力最强，其次是尖孢镰刀菌，最后是茄病镰刀菌，同时在所有测试真菌中，DON毒素的分泌量均高于FUM。β-D-葡聚糖生物聚合物对FUM和DON的吸附效果优于粘土和丙酸钙。在体内，用β-D-葡聚糖生物聚合物处理大豆种子可降低由轮枝镰刀菌人工接种的种子中FUM和DON毒素的水平。与未处理的种子相比，β-D-葡聚糖处理对种子营养成分的影响也较小。总之，本研究发现了一种新方法，可将饲料中的镰刀菌霉菌毒素降低到允许的安全限度，同时保持这些原料的营养价值。
