Liao Xiaolin, Tian Hua, Zhu Bin, Chang Jianbo
a Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources , Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences , Wuhan 430079 , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):328-9. doi: 10.3109/19401736.2014.892101. Epub 2014 Mar 24.
The complete mitochondrial genome of Chinese sturgeon (Acipenser sinensis) was determined by direct sequencing of PCR products. The Chinese sturgeon mitochondrial DNA is a circular molecule (16,688 bp in length) with the typical gene arrangement of vertebrate mtDNA, containing 13 protein-coding genes, two ribosomal RNA and 22 transfer RNA genes, and a non-coding control region. Its control region contains 4.5 copies of unit with 82 bp long at 5' end, which has been reported before for this species. Phylogenetic tree based on 13 protein-coding genes confirmed that the complete mtDNA sequence of Chinese sturgeon was reported here for the first time.
通过对聚合酶链反应（PCR）产物进行直接测序，测定了中华鲟（Acipenser sinensis）的完整线粒体基因组。中华鲟线粒体DNA是一个环状分子（长度为16,688碱基对），具有脊椎动物线粒体DNA典型的基因排列，包含13个蛋白质编码基因、两个核糖体RNA基因和22个转运RNA基因，以及一个非编码控制区。其控制区在5'端含有4.5个长度为82碱基对的单元拷贝，该物种此前已有相关报道。基于13个蛋白质编码基因构建的系统发育树证实，本文首次报道了中华鲟完整的线粒体DNA序列。
