Lee Jae-Bong, Kim Hyangmi, Park Doo-Sang, Yang Jae-Hyeong, Chun Young-Yull, Lee Kang Hyun, Bae Kyung Sook
Dokdo Fisheries Research Center, National Fisheries Research and Development Institute, Daejeon, Republic of Korea.
Antonie Van Leeuwenhoek. 2014 May;105(5):987-94. doi: 10.1007/s10482-014-0158-8. Epub 2014 Mar 25.
A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, D-17(T), was isolated from mud flats in the Yellow Sea in Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain D-17(T) belongs to the genus Pseudoruegeria and it shared 97.5 % similarity with the type strain of Pseudoruegeria haliotis WM67(T). The sequence similarities with Pseudoruegeria litimaris HD-43(T) and Pseudoruegeria aquimaris SW-255(T) were 96.9 and 96.1 %, respectively. Strain D-17(T) was found to grow with 0.5-6 % (w/v) NaCl, at 20-30 °C, and at pH 6.5-8.0. Strain D-17(T) was determined to contain Q-10 as the predominant ubiquinone and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, as defined by the MIDI system) as the major fatty acids. The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified lipid and four unidentified phospholipids. The DNA G+C content was determined to be 63.6 mol%. The DNA-DNA relatedness with P. haliotis WM67(T) was 32.5 %. The differential phenotypic properties revealed that strain D-17(T) can be separated from other Pseudoruegeria species. Based on the data presented in this study, strain D-17(T) represents a novel species, for which the name Pseudoruegeria limi sp. nov. is proposed. The type strain is D-17(T) (=KCTC 32460(T) =JCM 19487(T)).
从韩国黄海泥滩中分离出一株革兰氏阴性、需氧、无运动性的杆状细菌菌株D-17(T)。基于16S rRNA基因序列构建的系统发育树表明，菌株D-17(T)属于假鲁杰氏菌属，与鲍假鲁杰氏菌WM67(T)模式菌株的相似性为97.5%。与滨海假鲁杰氏菌HD-43(T)和海生假鲁杰氏菌SW-255(T)的序列相似性分别为96.9%和96.1%。发现菌株D-17(T)可在含0.5-6%（w/v）NaCl、20-30°C以及pH 6.5-8.0的条件下生长。经测定，菌株D-17(T)以Q-10作为主要泛醌，以特征性脂肪酸8（由MIDI系统定义的C18:1 ω7c和/或C18:1 ω6c）作为主要脂肪酸。主要极性脂被鉴定为磷脂酰甘油、双磷脂酰甘油、一种未鉴定的氨基脂、一种未鉴定的糖脂、一种未鉴定的脂类以及四种未鉴定的磷脂。DNA G+C含量经测定为63.6 mol%。与鲍假鲁杰氏菌WM67(T)的DNA-DNA相关性为32.5%。不同的表型特性表明菌株D-17(T)可与其他假鲁杰氏菌属物种区分开来。基于本研究提供的数据，菌株D-17(T)代表一个新物种，为此提议将其命名为黄海假鲁杰氏菌（Pseudoruegeria limi sp. nov.）。模式菌株为D-17(T)（=KCTC 32460(T)=JCM 19487(T)）。
