Baum Marc M, Gunawardana Manjula, Webster Paul
Department of Chemistry, Oak Crest Institute of Science, 2275 E Foothill Boulevard, Pasadena, CA, 91107, USA,
Methods Mol Biol. 2014;1147:85-103. doi: 10.1007/978-1-4939-0467-9_7.
Unraveling the complex ecology of the vaginal biofilm microbiome relies on a number of complementary techniques. Here, we describe the experimental approaches for studying vaginal microbial biofilm samples with a focus on specimen preparation for subsequent analysis. The techniques include fluorescence microscopy, fluorescence in situ hybridization, and scanning and transmission electron microscopy. Isolation of microbial DNA and RNA from these samples is covered along with a brief discussion of chemical analysis methods.
揭示阴道生物膜微生物群的复杂生态依赖于多种互补技术。在此，我们描述了研究阴道微生物生物膜样本的实验方法，重点是用于后续分析的样本制备。这些技术包括荧光显微镜、荧光原位杂交以及扫描和透射电子显微镜。文中还介绍了从这些样本中分离微生物DNA和RNA的方法，并简要讨论了化学分析方法。
