Romano V, Bosco P, Rocchi M, Costa G, Leube R E, Franke W W, Romeo G
Laboratorio di Genetica Molecolare, Istituto G. Gaslini, Genova, Italy.
Cytogenet Cell Genet. 1988;48(3):148-51. doi: 10.1159/000132612.
The chromosomal location of representative members of the type I and type II subfamilies of the cytokeratin multigene family was determined using specific cDNA probes in Southern blot hybridization with DNA from somatic cell hybrids. Our results show that the gene encoding human type II cytokeratin 4 resides on chromosome 12 and that encoding type I cytokeratin 15 is located on chromosome 17. The results indicate that cytokeratins are not concentrated in only one cluster. The possibility of the existence of separate type I and type II cytokeratin gene clusters is discussed.
利用特异性cDNA探针与体细胞杂种的DNA进行Southern印迹杂交，确定了细胞角蛋白多基因家族I型和II型亚家族代表性成员的染色体定位。我们的结果表明，编码人类II型细胞角蛋白4的基因位于12号染色体上，编码I型细胞角蛋白15的基因位于17号染色体上。结果表明，细胞角蛋白并不只集中在一个簇中。本文讨论了存在独立的I型和II型细胞角蛋白基因簇的可能性。
