活性I型DNA拓扑异构酶基因定位于人类染色体20q11.2 - 13.1，两个假基因分别定位于染色体1q23 - 24和22q11.2 - 13.1。
Localization of the active type I DNA topoisomerase gene on human chromosome 20q11.2-13.1, and two pseudogenes on chromosomes 1q23-24 and 22q11.2-13.1.
作者信息
Kunze N, Yang G C, Jiang Z Y, Hameister H, Adolph S, Wiedorn K H, Richter A, Knippers R
机构信息
Fakultät für Biologie der Universität, Konstanz, Federal Republic of Germany.
出版信息
Hum Genet. 1989 Dec;84(1):6-10. doi: 10.1007/BF00210661.
Different subfragments of a cDNA coding for DNA topoisomerase I were used as probes to determine the chromosomal localization of topoisomerase I sequences in human cells. Southern blotting of restricted DNA from a panel of rodent-human somatic cell hybrids revealed the localization of the complete gene on chromosome 20 and the presence of two truncated topoisomerase I pseudogene sequences on chromosomes 1 and 22. In situ chromosome hybridization experiments confirmed these results showing the location of the complete gene on band q11.2-13.1 of chromosome 20, and the location of the pseudogene sequences on band q23-24 of chromosome 1 and q11.2-13.1 of chromosome 22.
编码DNA拓扑异构酶I的cDNA的不同亚片段被用作探针，以确定人类细胞中拓扑异构酶I序列的染色体定位。对一组啮齿动物-人类体细胞杂种的限制性DNA进行Southern印迹分析，结果显示完整基因定位于20号染色体，并且在1号和22号染色体上存在两个截短的拓扑异构酶I假基因序列。原位染色体杂交实验证实了这些结果，显示完整基因位于20号染色体q11.2-13.1带，假基因序列分别位于1号染色体q23-24带和22号染色体q11.2-13.1带。
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