大麦黄矮病毒基因组RNA的序列与结构
Sequence and organization of barley yellow dwarf virus genomic RNA.
作者信息
Miller W A, Waterhouse P M, Gerlach W L
机构信息
CSIRO Division of Plant Industry, Canberra, Australia.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):6097-111. doi: 10.1093/nar/16.13.6097.
Abstract
The nucleotide sequence of the genomic RNA of barley yellow dwarf virus, PAV serotype was determined, except for the 5'-terminal base, and its genome organization deduced. The 5,677 nucleotide genome contains five large open reading frames (ORFs). The genes for the coat protein (1) and the putative viral RNA-dependent RNA polymerase were identified. The latter shows a striking degree of similarity to that of carnation mottle virus (CarMV). By comparison with corona- and retrovirus RNAs, it is proposed that a translational frameshift is involved in expression of the polymerase. An ORF encoding an Mr 49,797 protein (50K ORF) may be translated by in-frame readthrough of the coat protein stop codon. The coat protein, an overlapping 17K ORF, and a 3'6.7K ORF are likely to be expressed via subgenomic mRNAs.
摘要
除5'-末端碱基外，测定了大麦黄矮病毒PAV血清型基因组RNA的核苷酸序列，并推导了其基因组结构。这个5677个核苷酸的基因组包含五个大的开放阅读框（ORF）。鉴定出了外壳蛋白基因（1）和假定的病毒RNA依赖的RNA聚合酶基因。后者与香石竹斑驳病毒（CarMV）的聚合酶基因有显著的相似性。通过与冠状病毒和逆转录病毒RNA比较，推测聚合酶的表达涉及翻译移码。一个编码Mr 49,797蛋白（50K ORF）的ORF可能通过外壳蛋白终止密码子的框内通读进行翻译。外壳蛋白、一个重叠的17K ORF和一个3' 6.7K ORF可能通过亚基因组mRNA进行表达。
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本文引用的文献
1
Transcription of transposable element Activator (Ac) of Zea mays L.玉米转座因子 Activator（Ac）的转录
EMBO J. 1987 Jun;6(6):1555-63. doi: 10.1002/j.1460-2075.1987.tb02400.x.
2
A new computer method for the storage and manipulation of DNA gel reading data.一种用于存储和处理DNA凝胶读数数据的新型计算机方法。
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
3
An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.一个用于比较和比对核酸及氨基酸序列的交互式图形程序。
Nucleic Acids Res. 1982 May 11;10(9):2951-61. doi: 10.1093/nar/10.9.2951.
4
Nucleotide sequence of tobacco mosaic virus RNA.烟草花叶病毒RNA的核苷酸序列。
Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818-22. doi: 10.1073/pnas.79.19.5818.
5
The neural signal for skin indentation depth. I. Changing indentations.皮肤压痕深度的神经信号。I. 改变压痕
J Neurosci. 1983 Aug;3(8):1572-85. doi: 10.1523/JNEUROSCI.03-08-01572.1983.
6
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
7
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Eur J Biochem. 1984 Sep 3;143(2):277-84. doi: 10.1111/j.1432-1033.1984.tb08370.x.
8
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9
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10
Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses.植物、动物和细菌病毒的RNA依赖性聚合酶的一级结构比较。
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