Miller W A, Waterhouse P M, Gerlach W L
CSIRO Division of Plant Industry, Canberra, Australia.
Nucleic Acids Res. 1988 Jul 11;16(13):6097-111. doi: 10.1093/nar/16.13.6097.
The nucleotide sequence of the genomic RNA of barley yellow dwarf virus, PAV serotype was determined, except for the 5'-terminal base, and its genome organization deduced. The 5,677 nucleotide genome contains five large open reading frames (ORFs). The genes for the coat protein (1) and the putative viral RNA-dependent RNA polymerase were identified. The latter shows a striking degree of similarity to that of carnation mottle virus (CarMV). By comparison with corona- and retrovirus RNAs, it is proposed that a translational frameshift is involved in expression of the polymerase. An ORF encoding an Mr 49,797 protein (50K ORF) may be translated by in-frame readthrough of the coat protein stop codon. The coat protein, an overlapping 17K ORF, and a 3'6.7K ORF are likely to be expressed via subgenomic mRNAs.
除5'-末端碱基外，测定了大麦黄矮病毒PAV血清型基因组RNA的核苷酸序列，并推导了其基因组结构。这个5677个核苷酸的基因组包含五个大的开放阅读框（ORF）。鉴定出了外壳蛋白基因（1）和假定的病毒RNA依赖的RNA聚合酶基因。后者与香石竹斑驳病毒（CarMV）的聚合酶基因有显著的相似性。通过与冠状病毒和逆转录病毒RNA比较，推测聚合酶的表达涉及翻译移码。一个编码Mr 49,797蛋白（50K ORF）的ORF可能通过外壳蛋白终止密码子的框内通读进行翻译。外壳蛋白、一个重叠的17K ORF和一个3' 6.7K ORF可能通过亚基因组mRNA进行表达。
