Roomi M Waheed, Roomi Nusrath Waheed, Kalinovsky Tatiana, Rath Matthias, Niedzwiecki Aleksandra
Dr. Rath Research Institute, Santa Clara, CA 95050, USA.
Exp Ther Med. 2014 Apr;7(4):987-989. doi: 10.3892/etm.2014.1518. Epub 2014 Feb 3.
Amiodarone (Amio), a potent anti-arrhythmic drug, is associated with life-threatening pulmonary toxicity involving fibroses and inflammation. A unique nutrient mixture (NM) consisting of lysine, proline, ascorbic acid, -acetyl cysteine and green tea extract has previously been shown to exhibit a broad spectrum of pharmacological, therapeutic, cardiovascular and chemopreventive properties. The present study was undertaken to determine whether the NM exhibits preventive effects on Amio-induced cardiac toxicity. Six-week-old male BALB/c mice were divided into four groups (A-D) of six animals per group. Mice in groups A and C were fed a regular diet for three weeks, while the diets of the mice in groups B and D were supplemented with 1% NM during that period. After three weeks, the mice in groups C and D received daily Amio injections of 50 mg/kg body weight intraperitoneally for 4 days, whilst those in groups A and B received saline alone. At 24 h after the final dose, mice were sacrificed, blood was withdrawn and serum was collected for clinical chemistry of the heart enzymes creatine phosphokinase (CPK) and aspartate aminotransferase (AST). In addition, livers, kidneys, hearts and lungs were excised and weighed. No significant differences in weight gain were identified among the groups and liver, kidney, heart and lung weights were comparable in all four groups. Administration of Amio to group C resulted in a significant increase in serum CPK levels, whereas in NM-fed group D, the CPK levels were comparable to those in the saline injection groups, A and B. Amio administration also resulted in a significant increase in serum AST levels in group C, but not in the group D animals which exhibited similar levels to those of groups A and B. Therefore, the results indicate that NM has the potential to protect against Amio-induced cardiac toxicity.
胺碘酮（Amio）是一种强效抗心律失常药物，与包括纤维化和炎症在内的危及生命的肺部毒性有关。一种由赖氨酸、脯氨酸、抗坏血酸、N-乙酰半胱氨酸和绿茶提取物组成的独特营养混合物（NM）此前已被证明具有广泛的药理、治疗、心血管和化学预防特性。本研究旨在确定NM是否对胺碘酮诱导的心脏毒性具有预防作用。将六周龄雄性BALB/c小鼠分为四组（A-D），每组六只动物。A组和C组的小鼠连续三周喂食常规饮食，而在此期间B组和D组小鼠的饮食中添加1%的NM。三周后，C组和D组的小鼠每天腹腔注射50mg/kg体重的胺碘酮，持续4天，而A组和B组的小鼠仅注射生理盐水。在最后一剂后24小时，处死小鼠，采集血液并收集血清用于检测心脏酶肌酸磷酸激酶（CPK）和天冬氨酸转氨酶（AST）的临床化学指标。此外，切除肝脏、肾脏、心脏和肺并称重。各组之间体重增加无显著差异，且所有四组的肝脏、肾脏、心脏和肺重量相当。给C组小鼠施用胺碘酮导致血清CPK水平显著升高，而在喂食NM的D组中，CPK水平与生理盐水注射组A和B相当。施用胺碘酮还导致C组血清AST水平显著升高，但D组动物未出现这种情况，D组的AST水平与A组和B组相似。因此，结果表明NM有可能预防胺碘酮诱导的心脏毒性。
