Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Acta Vet Scand. 2014 Mar 26;56(1):18. doi: 10.1186/1751-0147-56-18.
The aim of the present study was to report a novel developmental abnormality in a cloned dog. A fibroblast cell line was established from an 8-year-old male German shepherd dog. In vivo matured oocytes were retrieved from a large breed dog, and the nucleus was removed from each oocyte. A donor cell was injected into an enucleated oocyte, and the oocyte-cell couplet was fused electrically. After chemical activation, the resulting embryos were transferred into a naturally estrus-synchronized recipient dog, and two cloned pups were delivered by Cesarean section 60 days later. One cloned pup (Clone 1) was healthy, but the other (Clone 2) had a birth weight of only 320 g and cleft palate, failure of preputial closure at the ventral distal part, and persistent penile frenulum. Clone 2 was raised by stomach feeding until Day 40 after birth, where palatoplasty was performed. The abnormalities in external genitalia in Clone 2 resulted in persistent penile extrusion that was surgically corrected. This complex developmental abnormality has not been reported in dogs previously.
本研究旨在报道一例克隆犬的新型发育异常。从一只 8 岁雄性德国牧羊犬中建立了一个成纤维细胞系。从一只大型犬中采集了体内成熟的卵母细胞，并从每个卵母细胞中去除了核。将供体细胞注入去核卵母细胞中，用电融合使卵母细胞-细胞对融合。化学激活后，将得到的胚胎移植到自然发情同步的受体犬中，60 天后通过剖腹产产下了两只克隆犬。一只克隆犬（克隆 1）健康，但另一只（克隆 2）出生体重仅为 320 克，存在腭裂、阴茎腹侧远端包皮闭合不全和持续的阴茎系带。克隆 2 通过胃饲喂养至出生后第 40 天，然后进行了腭裂修复术。克隆 2 的外生殖器异常导致持续的阴茎突出，需要手术矫正。这种复杂的发育异常在犬中以前没有报道过。
