Sánchez J, Cortés-Hernández D A, Escobedo-Bocardo J C, Jasso-Terán R A, Zugasti-Cruz A
Cinvestav-Unidad Saltillo, Ave. Industria Metalúrgica No. 1062, Parque Industrial Saltillo-Ramos Arizpe, CP 25900, Ramos Arizpe, Coah, México,
J Mater Sci Mater Med. 2014 Oct;25(10):2237-42. doi: 10.1007/s10856-014-5197-1. Epub 2014 Mar 27.
Hyperthermia is one of the most recents therapies for cancer treatment using particles with nanometric size and appropriate magnetic properties for destroying cancer cells. Magnetic nanoparticles (MNP's) of Fe-Ga and synthesized using a polycondensation reaction by sol-gel method were obtained. MNP's of Fe(1.4)Ga(1.6)O(4) that possess an inverse spinel structure were identified by X-Ray Diffraction, Transmission Electron Microscopy, Scanning Electron Microscopy and Energy Dispersive Spectroscopy. The results showed that the MNP's are composed only by Fe, Ga and O and their size is between 15 and 20 nm. The magnetic properties measured by Vibration Sample Magnetometry demonstrated a saturation magnetization value of 37.5 emu/g. To induce the MNP's bioactivity, a biomimetic method was used which consisted in the immersion of MNP's in a Simulated Body Fluid (SBF) for different periods of time (7, 14 and 21 d) along with a wollastonite disk. The formation of a bioactive layer, which closely resembles that formed on the existing bioactive systems and with a Ca/P atomic ratio within a range of 1.37-1.73 was observed on the MNP's. Cytotoxicity of MNP's was evaluated by in vitro hemolysis testing using human red blood cells at concentrations between 0.25 and 6.0 mg/mL. It was found that the MNP's were not cytotoxic at none of the concentrations used. The results indicate that Fe-Ga MNP's are potential materials for cancer treatment of both hard and soft tissue by hyperthermia and drug carriers, among other applications.
热疗是癌症治疗中最新的疗法之一，它使用具有纳米尺寸和适当磁性的粒子来破坏癌细胞。通过溶胶 - 凝胶法的缩聚反应获得了Fe - Ga磁性纳米粒子（MNP）。通过X射线衍射、透射电子显微镜、扫描电子显微镜和能量色散光谱鉴定了具有反尖晶石结构的Fe(1.4)Ga(1.6)O(4)磁性纳米粒子。结果表明，这些磁性纳米粒子仅由Fe、Ga和O组成，其尺寸在15至20纳米之间。通过振动样品磁强计测量的磁性表明饱和磁化强度值为37.5 emu/g。为了诱导磁性纳米粒子的生物活性，采用了一种仿生方法，即将磁性纳米粒子与硅灰石圆盘一起在模拟体液（SBF）中浸泡不同时间（7、14和21天）。在磁性纳米粒子上观察到形成了一层生物活性层，该层与现有生物活性系统上形成的层非常相似，且Ca/P原子比在1.37 - 1.73范围内。使用浓度在0.25至6.0 mg/mL之间的人红细胞通过体外溶血试验评估磁性纳米粒子的细胞毒性。发现在所用的任何浓度下，磁性纳米粒子都没有细胞毒性。结果表明，Fe - Ga磁性纳米粒子是通过热疗用于硬组织和软组织癌症治疗以及药物载体等其他应用的潜在材料。
