Carrieri Mariella, Bartolucci Giovanni Battista, Lee Taekhee, Barbero Ana, Harper Martin
1.Department of Cardiologic, Thoracic and Vascular Sciences, University of Padua, via Giustiniani, 2, Padua 35128, Italy;
2.Exposure Assessment Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505, USA.
Ann Occup Hyg. 2014 Jun;58(5):566-78. doi: 10.1093/annhyg/meu016. Epub 2014 Mar 26.
A novel high-performance liquid chromatographic/ultraviolet method was developed to detect lapachol (LP) and deoxylapachol (DLP) in wood dust as chemical markers of teak wood (a suspected human carcinogen). The specificity of this analysis was determined by noting the absence of LP and DLP in 12 other specimens of different woods belonging to the angiosperm family. The consistency was examined by analyzing teak from three different sources, where the percentages (wt/wt) of the chemicals ranged from 0.006 to 0.261 for LP and from 0.038 to 0.497 for DLP, respectively. Although the LP and DLP components of teak varied according to source, a very high correlation coefficient (r (2) > 0.98 always) was found between the content of the two markers in the bulk specimens and in bulk dust derived from them. The method was then applied to teak dust collected on polyvinylchloride filters from aerosol in an exposure chamber in the range of mass loadings between 0.03 and 3.65 mg, which corresponds to a dust exposure between 0.124 and 8.703 mg m(-3) for a sampling time of 2h. A field test was also carried out in a small factory where teak was used. A good correlation was confirmed between LP and DLP versus the dust collected on the filter in both cases. LP and DLP can be markers to estimate the true quantities of teak dust inhaled in a workplace with mixed wood dust, provided the results are matched to the content of LP and DLP in the bulk wood. LP and DLP have also been proposed as the agents responsible for allergic reaction to teak dust. Therefore, it would be useful to evaluate the exposure to these two substances even without a relationship to teak dust exposure.
开发了一种新型高效液相色谱/紫外方法，用于检测木屑中的拉帕醇（LP）和去氧拉帕醇（DLP），作为柚木（一种疑似人类致癌物）的化学标志物。通过观察12个属于被子植物科的其他不同木材样本中不存在LP和DLP，确定了该分析的特异性。通过分析来自三个不同来源的柚木来检验一致性，其中化学物质的百分比（重量/重量）对于LP分别为0.006至0.261，对于DLP为0.038至0.497。尽管柚木的LP和DLP成分因来源而异，但在大量样本及其衍生的大量灰尘中，两种标志物的含量之间始终发现非常高的相关系数（r²>0.98）。然后将该方法应用于在暴露室内的聚氯乙烯过滤器上收集的柚木灰尘，质量负荷范围为0.03至3.65mg，这对应于2小时采样时间的0.124至8.703mg m⁻³的粉尘暴露。还在一个使用柚木的小工厂进行了现场测试。在这两种情况下，LP和DLP与过滤器上收集的灰尘之间均确认有良好的相关性。如果结果与整块木材中LP和DLP的含量相匹配，LP和DLP可以作为估计在含有混合木屑的工作场所中吸入的柚木灰尘真实量的标志物。LP和DLP也被认为是对柚木灰尘过敏反应的原因。因此，即使与柚木灰尘暴露无关，评估对这两种物质的暴露也是有用的。
