Krogfelt K A, Meldal M, Klemm P
Department of Microbiology, Technical University of Denmark, Lyngby.
Microb Pathog. 1987 Jun;2(6):465-72. doi: 10.1016/0882-4010(87)90053-2.
Identification of antigenic determinants of K88 fimbriae was approached by immunization of rabbits with BSA conjugated synthetic peptides mimicking either a predicted common determinant or type specific determinants. Anti-peptide sera were assayed by ELISA and sandwich ELISA. It was shown that sera raised against the peptides corresponding to the K88ab and K88ad variants of the 213-219 segment were able to recognize the corresponding antigenic variant of the native fimbriae whereas the rest of the antisera did not to any significant degree react with intact fimbriae. In Western blots all anti-peptide sera were able to recognize the K88 proteins. Similarly in ELISA assays all raised sera showed affinity to denatured K88 proteins.
通过用与模拟预测的共同决定簇或型特异性决定簇的合成肽偶联的牛血清白蛋白免疫兔子，来鉴定K88菌毛的抗原决定簇。通过酶联免疫吸附测定（ELISA）和夹心ELISA检测抗肽血清。结果表明，针对213 - 219片段的K88ab和K88ad变体对应的肽产生的血清能够识别天然菌毛的相应抗原变体，而其余抗血清与完整菌毛没有明显反应。在蛋白质免疫印迹法中，所有抗肽血清都能够识别K88蛋白。同样，在ELISA测定中，所有产生的血清都显示出对变性K88蛋白的亲和力。
