Bosco F G, Bache M, Yang J, Chen C H, Hwu E-T, Lin Q, Boisen A
Department of Micro- and Nanotechnology, Technical University of Denmark, Lyngby 2800, Denmark.
Department of Mechanical Engineering, Columbia University, New York 10027, NY, United States.
Sens Actuators A Phys. 2013 Jun 1;195:154-159. doi: 10.1016/j.sna.2012.06.030.
A plug-and-play CD-like platform is used to perform a statistical detection of platelet derived growth factor (PDGF) proteins through aptamer-based surface functionalization of multiple microcantilever arrays. When PDGF proteins bind to aptamer coatings, the cantilevers deflect. The deflection response is monitored by optical read-out units from a commercial DVD-ROM device. We report on the use of an improved sensing platform which facilitates measurements under continuous liquid flow and with temperature control. Also, the mechanical wobbling of the DVD-ROM platform has been minimized and the scanning system has been optimized in order to detect cantilever deflections in liquid with nanometer scale resolution. The capability of the sensing platform is demonstrated by detection of clinically relevant concentrations of PDGF proteins. We present statistical measurements on 100 microcantilevers at different concentrations of PDGF, ranging from 10 nM to 400 nM. Hereby it is possible to reliably characterize the averaged mechanical response of cantilevers as a function of protein concentration.
一种即插即用的类似光盘的平台用于通过基于适体的多个微悬臂梁阵列表面功能化来对血小板衍生生长因子（PDGF）蛋白进行统计检测。当PDGF蛋白与适体涂层结合时，悬臂梁会发生偏转。偏转响应由来自商用DVD-ROM设备的光学读出单元进行监测。我们报告了一种改进的传感平台的使用情况，该平台便于在连续液体流动和温度控制下进行测量。此外，DVD-ROM平台的机械摆动已降至最低，并且扫描系统已得到优化，以便在液体中以纳米级分辨率检测悬臂梁的偏转。通过检测临床相关浓度的PDGF蛋白证明了该传感平台的能力。我们对100个微悬臂梁在10 nM至400 nM的不同PDGF浓度下进行了统计测量。由此可以可靠地表征悬臂梁的平均机械响应与蛋白质浓度的函数关系。
