Mattei D, Berzins K, Wahlgren M, Udomsangpetch R, Perlmann P, Griesser H W, Scherf A, Müller-Hill B, Bonnefoy S, Guillotte M
Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.
Parasite Immunol. 1989 Jan;11(1):15-29. doi: 10.1111/j.1365-3024.1989.tb00645.x.
A gene encoding a previously undescribed antigen of Plasmodium falciparum has been isolated from a genomic expression library by use of a pool of human immune sera. Northern blot analysis indicated that the gene is expressed at the late stages of the intra-erythrocytic cycle. This antigen, 332, contains a series of degenerated amino acid repeats. Human antibodies affinity-purified on the 332 recombinant antigen reacted with a family of parasite proteins that are products of different genes. We identified antigens 11.1 and Pf155-RESA as members of this family and confirmed, using a human monoclonal antibody, the presence of cross-reacting determinants. The sequences of these antigens also share some structural homologies. The significance of this family of blood-stage antigens is discussed.
利用一组人免疫血清，从基因组表达文库中分离出了一个编码恶性疟原虫一种此前未描述抗原的基因。Northern印迹分析表明，该基因在红细胞内周期的晚期表达。这种抗原332含有一系列退化的氨基酸重复序列。在332重组抗原上亲和纯化的人抗体与一族寄生虫蛋白发生反应，这些蛋白是不同基因的产物。我们鉴定出抗原11.1和Pf155-RESA是该族的成员，并使用人单克隆抗体证实了交叉反应决定簇的存在。这些抗原的序列也有一些结构同源性。本文讨论了这一族血液期抗原的意义。
