Sonnleitner Sissy Therese, Fritz Josef, Bednarska Malgorzata, Baumgartner Raphaela, Simeoni Josef, Zelger Roland, Schennach Harald, Lass-Flörl Cornelia, Edelhofer Renate, Pfister Kurt, Milhakov Andrea, Walder Gernot
Dr Gernot Walder GmbH, Außervillgraten, Austria.
Transfusion. 2014 Jul;54(7):1725-32. doi: 10.1111/trf.12606. Epub 2014 Mar 28.
After malaria, babesiosis is the second most common transfusion-transmitted parasitic disease in the United States. In Europe, one reported transfusion case, concerning Babesia microti, occurred in Germany.
Due to the fact that Babesia spp. are present in Tyrolean ticks, the aim of this study is to assess the occurrence of immunoglobulin (Ig)G antibodies against the Babesia divergens complex, including B. divergens and Babesia venatorum (EU1), as well as B. microti by screening a representative collective of 988 blood donors from North and East Tyrol (Austria) with indirect immunofluorescence antibody test. Additionally, we investigated 206 local ixodid ticks for the presence of babesial DNA by polymerase chain reaction.
Seroprevalence data resulted in rates of 2.1% for IgG antibodies against the B. divergens complex and 0.6% against B. microti in Tyrolean blood donors. All sera could be confirmed by independent retesting. Our data indicate that cross-reactivity is high between B. divergens and B. venatorum and lower than 19.8% between B. divergens and B. microti.
This study shows that Babesia spp. are present in the Tyrols, which blood donors come into serologic contact with, and that we have to consider how to sustain blood product safety concerning this new challenge. Additionally, it is the first description of B. venatorum in the Tyrols, found in one Ixodes ricinus at the Italian border.
在美国，巴贝斯虫病是继疟疾之后第二常见的输血传播寄生虫病。在欧洲，德国报告了一例与微小巴贝斯虫有关的输血病例。
由于蒂罗尔蜱虫中存在巴贝斯虫属，本研究旨在通过间接免疫荧光抗体试验对来自奥地利北蒂罗尔和东蒂罗尔的988名献血者的代表性样本进行筛查，以评估针对分歧巴贝斯虫复合体（包括分歧巴贝斯虫和嗜吞噬细胞无形体（欧盟1型））以及微小巴贝斯虫的免疫球蛋白（Ig）G抗体的出现情况。此外，我们通过聚合酶链反应调查了206只当地硬蜱是否存在巴贝斯虫DNA。
血清学流行率数据显示，蒂罗尔献血者中针对分歧巴贝斯虫复合体的IgG抗体率为2.1%，针对微小巴贝斯虫的为0.6%。所有血清均可通过独立复测得到确认。我们的数据表明，分歧巴贝斯虫和嗜吞噬细胞无形体之间的交叉反应性很高，而分歧巴贝斯虫和微小巴贝斯虫之间的交叉反应性低于19.8%。
本研究表明，蒂罗尔地区存在献血者血清学接触的巴贝斯虫属，我们必须考虑如何应对这一新挑战以维持血液制品安全。此外，这是首次在蒂罗尔地区发现嗜吞噬细胞无形体，在意大利边境的一只蓖麻硬蜱中发现。
