Puget Sound Blood Center, Seattle, Washington; University of Washington School of Medicine, Seattle, Washington.
Transfusion. 2014 Sep;54(9):2283-91. doi: 10.1111/trf.12634. Epub 2014 Mar 28.
Platelet (PLT) concentrates (PCs) prepared from whole blood in the United States are made using the PLT-rich plasma method. The PCs must be made within 8 hours of blood collection and stored for only 5 days. In Europe and Canada, PCs are made using the buffy coat (BC) method from whole blood held overnight at 22 °C and storage times may be up to 7 days. Our studies were designed to determine how long BC PLTs can be stored in plasma or Plasmalyte while meeting the FDA's poststorage viability criteria.
Normal subjects donated whole blood that was stored at 22 °C for 22 ± 2 hours before preparation of BC PLTs. PLTs were stored for 5 to 8 days in either plasma or Plasmalyte concentrations of 65 or 80%. Radiolabeled autologous stored versus fresh PLT recoveries and survivals were assessed as well as poststorage in vitro assays.
BC PLTs stored in either plasma or 65% Plasmalyte met FDA poststorage PLT recovery criteria for 7 days but survivals for only 6 days, while storage in 80% Plasmalyte gave very poor results. Both stored PLT recoveries and survivals correlated with the same donor's fresh results, but the correlation was much stronger between recoveries than survivals. In vitro measures of extent of shape change, morphology score, and pH best predicted poststorage PLT recoveries, while annexin V binding best predicted PLT survivals.
BC PLTs stored in either plasma or 65% Plasmalyte meet FDA's poststorage viability criteria for 6 days.
美国从全血中制备的血小板（PLT）浓缩物（PCs）采用富含 PLT 的血浆方法制备。这些 PCs 必须在采血后 8 小时内制成，并只能储存 5 天。在欧洲和加拿大，使用全血在 22°C 下保存过夜的浓缩白细胞层（BC）方法制备 PCs，储存时间可达 7 天。我们的研究旨在确定在满足 FDA 储存后活力标准的情况下，BC PLT 可以在血浆或 Plasmalyte 中储存多长时间。
正常受试者捐献全血，在制备 BC PLT 前在 22°C 下储存 22±2 小时。PLT 在血浆或 Plasmalyte 浓度为 65 或 80%下储存 5 至 8 天。评估放射性标记的自体储存与新鲜 PLT 的恢复率和存活率以及储存后的体外检测。
在血浆或 65%Plasmalyte 中储存的 BC PLT 满足 FDA 储存后 7 天的 PLT 恢复标准，但仅满足 6 天的存活率标准，而在 80%Plasmalyte 中储存的效果很差。储存的 PLT 恢复率和存活率均与同一供体的新鲜结果相关，但恢复率之间的相关性比存活率更强。体外形态变化程度、形态评分和 pH 的测量最能预测储存后的 PLT 恢复率，而 Annexin V 结合最能预测 PLT 存活率。
在血浆或 65%Plasmalyte 中储存的 BC PLT 满足 FDA 储存后 6 天的活力标准。
