Division of Clinical Chemistry and Laboratory Haematology, Department of Medical Laboratory Diagnostics, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211A St., 50-556 Wrocław, Poland.
Lower Silesian Oncology, Pulmonology and Hematology Center, 12 Hirszfeld Square, 53-413 Wrocław, Poland.
Cells. 2024 Mar 13;13(6):506. doi: 10.3390/cells13060506.
The reasons for unfavorable changes in platelet concentrate (PC) quality during storage are not fully understood yet. We aimed to evaluate whether leukocytes and matrix metalloproteinases (MMPs) lead to a decrease in the quality of PCs and examine whether MMP inhibition will slow down the platelets' aging. Nine PCs were divided into three parts: (1) leukocyte-depleted (F) PCs, (2) PCs with no additional procedures (NF), and (3) PCs with the addition of an MMP inhibitor-doxycycline (D). Each PC was stored for 144 h, and a sample for testing was separated from each part on the day of preparation and after 24, 48, 72 and 144 h of storage. Blood morphological analysis, platelet aggregation, and the expression of activation markers were evaluated. MMP-2 and MMP-9 concentration, activity, and gene expression were assessed. Platelet aggregation decreased, and platelet activation marker expression increased during the storage. D concentrates showed the lowest level of platelet activation. In turn, leukocyte-depleted PCs showed the highest level of platelet activation in general. MMP-9 platelet activity was higher in leukocyte-containing concentrates at the end of the storage period. We concluded that the filtration process leads to a higher platelet activation level. The presence of doxycycline in PCs reduces the expression of the activation markers as compared to leukocyte-depleted concentrates.
血小板浓缩物（PC）在储存过程中质量变差的原因尚未完全阐明。我们旨在评估白细胞和基质金属蛋白酶（MMPs）是否会导致 PC 质量下降，并研究 MMP 抑制是否会减缓血小板衰老。将 9 份 PC 分成三组：（1）去白细胞（F）PC，（2）无附加操作的 PC（NF），和（3）添加 MMP 抑制剂强力霉素（D）的 PC。每份 PC 储存 144 小时，在制备当天和储存 24、48、72 和 144 小时后，从每个部分分离出一个用于测试的样本。评估血液形态学分析、血小板聚集和活化标志物的表达。评估 MMP-2 和 MMP-9 浓度、活性和基因表达。在储存过程中，血小板聚集减少，血小板活化标志物的表达增加。D 浓缩物表现出最低的血小板活化水平。反过来，去白细胞 PC 总体上表现出最高的血小板活化水平。在储存期末，含白细胞的浓缩物中 MMP-9 血小板活性更高。我们得出结论，过滤过程会导致更高的血小板活化水平。与去白细胞浓缩物相比，PC 中强力霉素的存在会降低活化标志物的表达。
