• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

考马斯亮蓝染色

Coomassie blue staining.

作者信息

Brunelle Julie L, Green Rachel

机构信息

Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

出版信息

Methods Enzymol. 2014;541:161-7. doi: 10.1016/B978-0-12-420119-4.00013-6.

DOI:10.1016/B978-0-12-420119-4.00013-6
PMID:24674070
Abstract

This protocol describes Coomassie brilliant blue staining, one of the most common methods of detecting proteins in polyacrylamide gels (PAGE).

摘要

本方案描述了考马斯亮蓝染色法,这是检测聚丙烯酰胺凝胶(PAGE)中蛋白质最常用的方法之一。

相似文献

1
Coomassie blue staining.考马斯亮蓝染色
Methods Enzymol. 2014;541:161-7. doi: 10.1016/B978-0-12-420119-4.00013-6.
2
Coomassie Brilliant Blue removal/disposal from gel destain and used gel stain in an environment-friendly manner.以环保方式从凝胶脱色液中去除/处理考马斯亮蓝以及处理用过的凝胶染色剂。
Methods Mol Biol. 2012;869:607-16. doi: 10.1007/978-1-61779-821-4_55.
3
Paper Adsorbents Remove Coomassie Blue from Gel Destain and Used Gel Stain in an Environment-Friendly Manner.纸质吸附剂以环保方式从凝胶脱色液中去除考马斯亮蓝并回收用过的凝胶染色剂。
Methods Mol Biol. 2018;1853:259-268. doi: 10.1007/978-1-4939-8745-0_30.
4
Environmentally safe removal/disposal of Coomassie Brilliant Blue from gel destain and used gel stain.安全环保地从凝胶脱色液和使用过的凝胶染色液中去除/处置考马斯亮蓝。
Anal Biochem. 2010 Sep 15;404(2):193-6. doi: 10.1016/j.ab.2010.05.022. Epub 2010 May 25.
5
Application of Neuhoff's optimized Coomassie brilliant blue G-250/ammonium sulfate/phosphoric acid protein staining to ultrathin polyacrylamide gels on polyester films.Neuhoff优化的考马斯亮蓝G-250/硫酸铵/磷酸蛋白质染色法在聚酯薄膜上超薄聚丙烯酰胺凝胶中的应用。
Electrophoresis. 1988 May;9(5):236-8. doi: 10.1002/elps.1150090510.
6
Rapid coomassie blue staining of protein gels.蛋白质凝胶的快速考马斯亮蓝染色
Cold Spring Harb Protoc. 2010 Apr;2010(4):pdb.prot5413. doi: 10.1101/pdb.prot5413.
7
A fluorescence-based Coomassie Blue protocol for two-dimensional gel-based proteomics.基于荧光考马斯亮蓝的二维凝胶蛋白质组学方法。
Biotechnol Lett. 2011 Jan;33(1):119-21. doi: 10.1007/s10529-010-0404-8. Epub 2010 Sep 30.
8
A convenient procedure for transfer blotting of coomassie blue stained proteins from PAGE gels to transparencies.一种将考马斯亮蓝染色的蛋白质从聚丙烯酰胺凝胶转移至透明胶片上的便捷方法。
Biotechniques. 1993 Jun;14(6):925-30.
9
Coomassie-Brilliant Blue staining of polyacrylamide gels.聚丙烯酰胺凝胶的考马斯亮蓝染色。
Methods Mol Biol. 2012;869:465-9. doi: 10.1007/978-1-61779-821-4_40.
10
Improved detection of lymphocyte membrane proteins in purified form and as a crude mixture using native and denaturing polyacrylamide gel electrophoresis by optimisation of coomassie brilliant blue and silver staining.通过优化考马斯亮蓝和银染,利用天然和变性聚丙烯酰胺凝胶电泳改进对纯化形式以及粗混合物形式的淋巴细胞膜蛋白的检测。
Electrophoresis. 1990 Jan;11(1):53-60. doi: 10.1002/elps.1150110112.

引用本文的文献

1
Small heat shock protein HSPB8 interacts with a pre-fibrillar TDP43 low complexity domain species to delay fibril formation.小热休克蛋白HSPB8与原纤维前TDP43低复杂性结构域物质相互作用,以延迟纤维形成。
bioRxiv. 2025 Jan 30:2025.01.28.635368. doi: 10.1101/2025.01.28.635368.
2
Protocol for identification of NEMF-mediated C-terminal extensions on mitochondrial nonstop proteins via customized MS/MS spectra database searching.通过定制的串联质谱(MS/MS)谱数据库搜索鉴定线粒体无终止密码子蛋白质上NEMF介导的C末端延伸的方案。
STAR Protoc. 2024 Dec 20;5(4):103366. doi: 10.1016/j.xpro.2024.103366. Epub 2024 Oct 11.
3
Loss of Lipooligosaccharide Synthesis in Produces Changes in Outer Membrane Vesicle Protein Content.
缺失脂寡糖合成导致外膜囊泡蛋白含量发生变化。
Int J Mol Sci. 2024 Aug 27;25(17):9272. doi: 10.3390/ijms25179272.
4
Vicia ervilia lectin (VEA) has an antibiofilm effect on both Gram-positive and Gram-negative pathogenic bacteria.野豌豆属植物凝集素(VEA)对革兰氏阳性和革兰氏阴性致病菌均具有抗生物膜作用。
Arch Microbiol. 2024 Aug 9;206(9):371. doi: 10.1007/s00203-024-04100-6.
5
Method for B Cell Receptor Enrichment in Malignant B Cells.恶性B细胞中B细胞受体富集的方法。
Cancers (Basel). 2024 Jun 26;16(13):2341. doi: 10.3390/cancers16132341.
6
A Novel Ophthalmic Solution Containing Glicopro Complex for the Treatment of Patients with Dry Eye Disease: Results from a Pilot Study.一种含格列科普复合物的新型眼科溶液治疗干眼症患者:一项初步研究的结果。
J Clin Med. 2024 Mar 1;13(5):1447. doi: 10.3390/jcm13051447.
7
Phosphatase and Pseudo-Phosphatase Functions of Phosphatase of Regenerating Liver 3 (PRL-3) Are Insensitive to Divalent Metals In Vitro.再生肝脏磷酸酶3(PRL-3)的磷酸酶和假磷酸酶功能在体外对二价金属不敏感。
ACS Omega. 2023 Aug 9;8(33):30578-30589. doi: 10.1021/acsomega.3c04095. eCollection 2023 Aug 22.
8
Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia.用于治疗单核细胞白血病的植物和植物细胞中表达的重组免疫毒素的设计、优化、生产和活性测试。
Bioengineered. 2023 Dec;14(1):2244235. doi: 10.1080/21655979.2023.2244235.
9
Investigating the Effects of Alltech Crop Science (ACS) Products on Plant Defence against Root-Knot Nematode Infestation.研究奥特奇作物科学(ACS)产品对植物抵御根结线虫侵染的影响。
Microorganisms. 2023 Jun 29;11(7):1700. doi: 10.3390/microorganisms11071700.
10
Antimicrobial Activity of Bee Venom Collected in Northern Peru.秘鲁北部采集的蜂毒的抗菌活性。
Antibiotics (Basel). 2023 Apr 19;12(4):779. doi: 10.3390/antibiotics12040779.