Pulavarty Anusha, Singh Ankit, Young Kira, Horgan Karina, Kakouli-Duarte Thomais
Molecular Ecology and Nematode Research Group, Department of Applied Science, enviroCORE, Kilkenny Road Campus, South East Technological University (SETU), R93 V960 Carlow, Ireland.
Alltech Bioscience Centre, A86 X006 Dunboyne, Ireland.
Microorganisms. 2023 Jun 29;11(7):1700. doi: 10.3390/microorganisms11071700.
Two formulations of Alltech Crop Science products (ACS), a proprietary blend of fermentation products and plant extracts with micronutrients (ACS5075), and a microbial based product (ACS3048), were tested to understand (1) their impact on the tomato plant immune response and (2) whether they are priming a resistance response in plants against root knot nematodes (RKN). Research findings reported previously indicate that tomato plants pre-treated with ACS5075 and ACS3048 were found less sensitive to infection. In the current study, the expression of six defence-related genes (, , , , and ), relative to a housekeeping gene, were monitored via RT-PCR. Results suggest that the treatment with ACS5075 enhanced and gene expression levels, both post- treatment and post-infection with . Reduced infestation that was reported in the previous study could be attributed to the increased expression of these genes in the ACS5075-treated plants. Tomato plants treated with ACS3048, but without RKN infection, also demonstrated higher levels of and gene expression. Subsequently, 2D-gel electrophoresis was performed to study the differential protein expression in leaf tissues of treated tomato plants in an effort to elucidate a possible mechanism of action for these products. Protein spot 1 was identified as 'disease resistance protein RPP13-like', protein spot 2 as 'phosphatidylinositol 4-phosphate 5-kinase 2', spot 3 as 'protein SABRE like' and protein spot 4 as 'uncharacterized protein'. Overall research findings indicate that the ACS products could be used as plant immunity-boosting agents, as they play a significant role in the expression of certain genes and proteins associated with plant defence.
对奥特奇作物科学公司（Alltech Crop Science，简称ACS）的两种产品配方进行了测试，其中一种是发酵产品与含有微量营养素的植物提取物的专利混合物（ACS5075），另一种是基于微生物的产品（ACS3048），以了解：（1）它们对番茄植株免疫反应的影响；（2）它们是否能引发植物对根结线虫（RKN）的抗性反应。先前报道的研究结果表明，用ACS5075和ACS3048预处理的番茄植株对感染的敏感性较低。在当前研究中，通过逆转录聚合酶链反应（RT-PCR）监测了六个与防御相关的基因（、、、、和）相对于一个管家基因的表达。结果表明，用ACS5075处理后，在接种后和感染后，和基因的表达水平均有所提高。先前研究中报道的侵染减少可能归因于这些基因在ACS5075处理的植株中表达增加。用ACS3048处理但未感染根结线虫的番茄植株也表现出较高水平的和基因表达。随后，进行了二维凝胶电泳，以研究处理过的番茄植株叶片组织中的差异蛋白质表达，从而阐明这些产品可能的作用机制。蛋白质斑点1被鉴定为“抗病蛋白RPP13样蛋白”，蛋白质斑点2为“磷脂酰肌醇4-磷酸5-激酶2”，斑点3为“类SABRE蛋白”，蛋白质斑点4为“未知蛋白”。总体研究结果表明，ACS产品可作为植物免疫增强剂，因为它们在与植物防御相关的某些基因和蛋白质的表达中发挥着重要作用。
