Department of Molecular and Cell Biomedicine, State Scientific Center of Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia; I.M. Sechenov First Moscow State Medical University, Moscow, Russia; and
Department of Molecular and Cell Biomedicine, State Scientific Center of Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia; Moscow Institute of Physics and Technology (State University), Moscow, Russia.
J Appl Physiol (1985). 2014 May 15;116(10):1315-23. doi: 10.1152/japplphysiol.00134.2014. Epub 2014 Mar 27.
The aim of the work was to analyze changes in the organization of the cortical cytoskeleton in fibers of the mouse soleus muscle, tibialis anterior muscle and left ventricular cardiomyocytes after completion of a 30-day space flight on board the BION-M1 biosatellite (Russia, 2013). The transversal stiffness of the cortical cytoskeleton of the cardiomyocytes and fibers of the skeletal muscles did not differ significantly within the study groups compared with the vivarium control group. The content of beta- and gamma-actin in the membranous fraction of proteins in the left ventricular cardiomyocytes did not differ significantly within all study groups and correlated with the transversal stiffness. A similar situation was revealed in fibers of the soleus muscle and tibialis anterior muscle. At the same time, the content of beta-actin in the cytoplasmic fraction of proteins was found to be decreased in all types of studied tissues compared with the control levels in the postflight group, with lowered beta-actin gene expression rates in the postflight group. After completion of the space flight, the content of alpha-actinin-4 was found to be reduced in the membranous fraction of proteins from the mouse cardiomyocytes, while its content in the cytoplasmic fraction of proteins did not change significantly. Furthermore, gene expression rates of this protein were decreased at the time of dissection (it was started after 13 h after landing). At the same time, the content of alpha-actinin-1 decreased in the membranous fraction and increased in the cytoplasmic fraction of proteins from the soleus muscle fibers.
本工作旨在分析 2013 年搭乘 BION-M1 号生物卫星（俄罗斯）完成 30 天太空飞行后，小鼠比目鱼肌、胫骨前肌纤维和左心室心肌细胞皮质细胞骨架的组织变化。与对照 vivarium 组相比，实验组心肌细胞和骨骼肌纤维皮质细胞骨架的横向刚性没有显著差异。左心室心肌细胞蛋白膜部分的β-和γ-肌动蛋白含量在所有实验组内均无显著差异，与横向刚性相关。比目鱼肌和胫骨前肌纤维也存在类似情况。与此同时，与飞行后组对照水平相比，所有类型研究组织的蛋白细胞质部分的β-肌动蛋白含量均降低，飞行后组的β-肌动蛋白基因表达率降低。太空飞行完成后，发现来自小鼠心肌细胞的蛋白膜部分的α-辅肌动蛋白-4 含量降低，而其蛋白细胞质部分含量无明显变化。此外，该蛋白的基因表达率在解剖时降低（在着陆后 13 小时开始）。与此同时，来自比目鱼肌纤维的蛋白膜部分的α-辅肌动蛋白-1 含量降低，而细胞质部分含量增加。
