Cell Biophysics Lab, State Scientific Center of the Russian Federation Institute of Biomedical Problems of the Russian Academy of Sciences, Khoroshevskoyoe shosse, 76a, Moscow, 123007, Russia.
I. M. Sechenov First Moscow State Medical University, 8-2 Trubetskaya St., Moscow, 119991, Russia.
Sci Rep. 2019 May 28;9(1):7974. doi: 10.1038/s41598-019-44468-w.
Under microgravity, the gene expression levels vary in different types of cells; however, the reasons for this have not been sufficiently studied. The aim of this work was to evaluate the methylation of CpG islands in the promoter regions of the genes encoding some cytoskeletal proteins, the total methylation and 5 hmC levels, and the levels of enzymes that regulate these processes in the testes, heart, and lungs in mice after a 30-day microgravity modeling by antiorthostatic suspension and after a subsequent 12-hour recovery as well as in the corresponding control group and identical groups treated with essential phospholipids. The obtained results indicate that under modeling microgravity in the examined tissues a decrease of cytoskeletal gene expression (mainly in the heart and lungs tissues) correlated with an increase in the CpG islands methylation and an increase of the expression (mainly in the testes tissue) - with a decrease of the CpG-methylation, despite of the fact that in the examined tissues took place a decrease of the content methylases and demethylases. But the deacetylase HDAC1 content increased in the heart and lungs tissues and decreased in the testes, letting us suggest its participation in the regulation of the methylation level under microgravity conditions.
在微重力条件下，不同类型的细胞中的基因表达水平会发生变化；然而，其原因尚未得到充分研究。本工作旨在评估模拟微重力（通过抗重力悬停） 30 天后以及随后的 12 小时恢复后，以及在相应的对照组和用必需磷脂处理的相同组中，编码某些细胞骨架蛋白的基因的启动子区域中的 CpG 岛的甲基化、总甲基化和 5-hmC 水平，以及调节这些过程的酶在小鼠的睾丸、心脏和肺部中的水平。所得结果表明，在检查的组织中模拟微重力时，细胞骨架基因表达的降低（主要在心脏和肺部组织中）与 CpG 岛甲基化的增加以及表达的增加（主要在睾丸组织中）相关联）- 伴随着 CpG-甲基化的降低，尽管在检查的组织中发生了甲基转移酶和去甲基化酶含量的降低。但是，心脏和肺部组织中的去乙酰化酶 HDAC1 含量增加，睾丸中的含量减少，这让我们推测其在微重力条件下参与调节甲基化水平。
