Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada
J Appl Physiol (1985). 2014 May 15;116(10):1272-80. doi: 10.1152/japplphysiol.00074.2014. Epub 2014 Mar 27.
Exercise training reduces systemic and adipose tissue inflammation. However, these beneficial effects seem to be largely tied to reductions in adipose tissue mass. The purpose of the present study was to determine if exercise training confers a protective effect against an acute inflammatory challenge. We hypothesized that the induction of inflammatory markers, such as interleukin 6 (IL-6), suppressor of cytokine signaling 3 (SOCS3), and TNF-α by the beta-3 adrenergic agonist CL 316,243 would be reduced in adipose tissue from trained mice and this would be associated with reductions in transient receptor potential cation channel 4 (TRPV4), a protein recently shown to regulate the expression of proinflammatory cytokines. Exercise training (4 wk of treadmill running, 1 h/day, 5 days/wk) increased markers of skeletal muscle mitochondrial content and the induction of PPAR-gamma coactivator 1 alpha in epididymal adipose tissue. The mRNA expression of IL-6, SOCS3, and TNFα were not different in subcutaneous and epididymal adipose tissue from sedentary and trained mice; however, the CL 316,243-mediated induction of these genes was attenuated ∼50% in epididymal adipose tissue from trained mice as were increases in plasma IL-6. The effects of training were not explained by reductions in lipolytic responsiveness, but were associated with decreases in TRPV4 protein content. These results highlight a previously unappreciated anti-inflammatory effect of exercise training on adipose tissue immunometabolism and underscores the value of assessing adipose tissue inflammation in the presence of an inflammatory insult.
运动训练可减少全身和脂肪组织的炎症。然而，这些有益的效果似乎在很大程度上与脂肪组织质量的减少有关。本研究的目的是确定运动训练是否对急性炎症挑战具有保护作用。我们假设，β-3 肾上腺素能激动剂 CL 316,243 诱导的炎症标志物，如白细胞介素 6（IL-6）、细胞因子信号转导抑制因子 3（SOCS3）和 TNF-α，在经过训练的小鼠的脂肪组织中会减少，并且这与瞬时受体电位阳离子通道 4（TRPV4）的减少有关，TRPV4 是一种最近被证明可调节促炎细胞因子表达的蛋白质。运动训练（4 周跑步机跑步，每天 1 小时，每周 5 天）增加了附睾脂肪组织中骨骼肌线粒体含量和过氧化物酶体增殖物激活受体-γ共激活因子 1α的诱导标志物。在安静和训练的小鼠的皮下和附睾脂肪组织中，IL-6、SOCS3 和 TNFα 的 mRNA 表达没有差异；然而，在训练的小鼠的附睾脂肪组织中，CL 316,243 介导的这些基因的诱导减少了约 50%，同时血浆 IL-6 也增加。训练的影响不能用脂肪分解反应性的降低来解释，而是与 TRPV4 蛋白含量的降低有关。这些结果突出了运动训练对脂肪组织免疫代谢的抗炎作用，强调了在存在炎症刺激时评估脂肪组织炎症的价值。
