Chen Neng, Cheng Jinbo, Zhou Lingmei, Lei Ting, Chen Lihua, Shen Qiang, Qin Liqiang, Wan Zhongxiao
Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Renai Road 199, Dushuhu Higher Education Town, Suzhou, China, 215123.
Suzhou Industrial Park Center Disease Control and Prevention, 58 Suqian Road, Suzhou, 215123, People's Republic of China.
J Physiol Biochem. 2015 Dec;71(4):733-42. doi: 10.1007/s13105-015-0437-5. Epub 2015 Sep 30.
To explore the effects of rutin and exercise on high-fat diet (HFD)-induced disrupted lipolytic signaling, adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) signaling, transient receptor potential cation channel subfamily V member 4 (TRPV4) and its associated protein expression, and whether depot-specific effects existed. C57BL/6J mice were randomized into five groups: chow group, HFD, HFD plus rutin intervention group (HR), HFD combined with treadmill running group (HE), and HFD combined with treadmill running and rutin intervention group (HRE). At the end of the 16-week intervention, lipolytic markers, AMPK signaling pathways, TRPV4, and peroxisome proliferator-activated receptor gamma coactivator 1α + β (PGC-1α + β) from adipose tissue were measured by western blotting. In epididymal adipose tissue, HFD resulted in significant reduction in the phosphorylation of hormone sensitive lipase at serine660 (p-HSL660), perilipin A, phosphoenolpyruvate carboxykinase (PEPCK), p-AMPK, and p-acetyl-CoA carboxylase (ACC) protein expression. Exercise intervention and exercise plus rutin completely restored p-HSL660, perilipin A, PEPCK, p-AMPK, and p-ACC protein expression to normal level. HFD and HR groups have reduced expression of PGC-1α + β, exercise, and exercise plus rutin completely restored PGC-1α + β expression to normal level. In subcutaneous adipose tissue, HFD elevated TRPV4, exercise, and exercise plus rutin completely reduced TRPV4 to normal level. HR, HE, and HRE group have increased PGC-1α + β. In conclusion, depot-specific effects existed in regards to how rutin and exercise affect lipolytic signaling and p-AMPK, as well as TRPV4 and PGC-1α + β expression.
为探究芦丁和运动对高脂饮食（HFD）诱导的脂解信号通路破坏、5'-单磷酸腺苷（AMP）激活的蛋白激酶（AMPK）信号通路、瞬时受体电位阳离子通道亚家族V成员4（TRPV4）及其相关蛋白表达的影响，以及是否存在特定部位效应。将C57BL/6J小鼠随机分为五组：正常饮食组、高脂饮食组、高脂饮食加芦丁干预组（HR）、高脂饮食结合跑步机跑步组（HE）、高脂饮食结合跑步机跑步及芦丁干预组（HRE）。在16周干预结束时，通过蛋白质印迹法检测脂肪组织中的脂解标志物、AMPK信号通路、TRPV4以及过氧化物酶体增殖物激活受体γ辅激活因子1α + β（PGC-1α + β）。在附睾脂肪组织中，高脂饮食导致丝氨酸660位点的激素敏感性脂肪酶（p-HSL660）、围脂滴蛋白A、磷酸烯醇式丙酮酸羧激酶（PEPCK）、p-AMPK和p-乙酰辅酶A羧化酶（ACC）蛋白表达显著降低。运动干预以及运动加芦丁可使p-HSL660、围脂滴蛋白A、PEPCK、p-AMPK和p-ACC蛋白表达完全恢复至正常水平。高脂饮食组和HR组的PGC-1α + β表达降低，运动以及运动加芦丁可使PGC-1α + β表达完全恢复至正常水平。在皮下脂肪组织中，高脂饮食使TRPV4升高，运动以及运动加芦丁可使TRPV4完全降低至正常水平。HR组、HE组和HRE组的PGC-1α + β增加。总之，芦丁和运动对脂解信号通路和p-AMPK以及TRPV4和PGC-1α + β表达的影响存在特定部位效应。
