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1
Total synthesis of a functional designer eukaryotic chromosome.功能设计真核染色体的全合成。
Science. 2014 Apr 4;344(6179):55-8. doi: 10.1126/science.1249252. Epub 2014 Mar 27.
2
Total synthesis of a eukaryotic chromosome: Redesigning and SCRaMbLE-ing yeast.真核染色体的全合成：对酵母进行重新设计与SCRaMbLE操作
Bioessays. 2014 Sep;36(9):855-60. doi: 10.1002/bies.201400086. Epub 2014 Jul 22.
3
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.酿酒酵母第八条染色体的完整核苷酸序列。
Science. 1994 Sep 30;265(5181):2077-82. doi: 10.1126/science.8091229.
4
Synthetic chromosome arms function in yeast and generate phenotypic diversity by design.人工合成染色体臂在酵母中具有功能，并通过设计产生表型多样性。
Nature. 2011 Sep 14;477(7365):471-6. doi: 10.1038/nature10403.
5
Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: characterization of the 54 kb right terminal CDC15-FLO1-PHO11 region.酿酒酵母1号染色体DNA的分子克隆：54kb右末端CDC15 - FLO1 - PHO11区域的特征分析
Yeast. 1997 Oct;13(13):1251-63. doi: 10.1002/(sici)1097-0061(199710)13:13<1251::aid-yea174>3.3.co;2-6.
6
Verification of a new gene on Saccharomyces cerevisiae chromosome III.酿酒酵母第三条染色体上一个新基因的验证
Yeast. 2003 Jun;20(8):731-8. doi: 10.1002/yea.996.
7
Life with 6000 genes.拥有6000个基因的生命。
Science. 1996 Oct 25;274(5287):546, 563-7. doi: 10.1126/science.274.5287.546.
8
SynV and SynX: A story more than DNA synthesis.合成病毒V和合成病毒X：一个不仅仅关乎DNA合成的故事。
Sci China Life Sci. 2017 May;60(5):558-560. doi: 10.1007/s11427-017-9039-6. Epub 2017 May 5.
9
3D organization of synthetic and scrambled chromosomes.合成染色体和随机排列染色体的三维结构
Science. 2017 Mar 10;355(6329). doi: 10.1126/science.aaf4597.
10
tRNA genes and retroelements in the yeast genome.酵母基因组中的转运RNA基因和反转录元件。
Nucleic Acids Res. 1998 Feb 1;26(3):689-96. doi: 10.1093/nar/26.3.689.
引用本文的文献
1
Scaling DNA engineering.扩展DNA工程。
Trends Biotechnol. 2025 May 28. doi: 10.1016/j.tibtech.2025.05.002.
2
Synthetic biology in plants.植物合成生物学
Plant Biotechnol (Tokyo). 2024 Sep 25;41(3):173-193. doi: 10.5511/plantbiotechnology.24.0630b.
3
Towards the first synthetic eukaryotic cell.迈向首个合成真核细胞。
Biosaf Health. 2024 Nov 15;6(6):376-382. doi: 10.1016/j.bsheal.2024.11.001. eCollection 2024 Dec.
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SHIP identifies genomic safe harbors in eukaryotic organisms using genomic general feature annotation.SHIP利用基因组一般特征注释识别真核生物中的基因组安全港。
Sci Rep. 2025 Feb 28;15(1):7193. doi: 10.1038/s41598-025-91249-9.
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Design and structure of overlapping regions in PCA via deep learning.通过深度学习实现主成分分析中重叠区域的设计与结构
Synth Syst Biotechnol. 2024 Dec 27;10(2):442-451. doi: 10.1016/j.synbio.2024.12.007. eCollection 2025 Jun.
6
Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome.酿酒酵母903 kb合成染色体synXVI的构建与迭代重新设计。
Nat Commun. 2025 Jan 20;16(1):841. doi: 10.1038/s41467-024-55318-3.
7
Recent advances in genome-scale engineering in and their applications.基因组规模工程的最新进展及其应用。 （你提供的原文“in and their applications”表述似乎不完整，推测完整表述可能是“Recent advances in genome-scale engineering in organisms and their applications”，这里按照推测完整后的内容进行了翻译，你可根据实际情况调整 ）
Eng Microbiol. 2023 Sep 15;4(1):100115. doi: 10.1016/j.engmic.2023.100115. eCollection 2024 Mar.
8
Link Between Individual Codon Frequencies and Protein Expression: Going Beyond Codon Adaptation Index.个体密码子频率与蛋白质表达之间的关系：超越密码子适应指数。
Int J Mol Sci. 2024 Oct 29;25(21):11622. doi: 10.3390/ijms252111622.
9
The design and engineering of synthetic genomes.合成基因组的设计与工程
Nat Rev Genet. 2025 May;26(5):298-319. doi: 10.1038/s41576-024-00786-y. Epub 2024 Nov 6.
10
Reaching New Heights in Genetic Code Manipulation with High Throughput Screening.高通量筛选助力基因密码操作技术新突破
Chem Rev. 2024 Nov 13;124(21):12145-12175. doi: 10.1021/acs.chemrev.4c00329. Epub 2024 Oct 17.
本文引用的文献
1
The Saccharomyces cerevisiae SCRaMbLE system and genome minimization.酿酒酵母SCRaMbLE系统与基因组最小化
Bioeng Bugs. 2012 May-Jun;3(3):168-71. doi: 10.4161/bbug.19543. Epub 2012 May 1.
2
Assembling large DNA segments in yeast.在酵母中组装大型DNA片段。
Methods Mol Biol. 2012;852:133-50. doi: 10.1007/978-1-61779-564-0_11.
3
Assembling DNA fragments by USER fusion.通过USER融合组装DNA片段。
Methods Mol Biol. 2012;852:77-95. doi: 10.1007/978-1-61779-564-0_7.
4
Synthetic chromosome arms function in yeast and generate phenotypic diversity by design.人工合成染色体臂在酵母中具有功能，并通过设计产生表型多样性。
Nature. 2011 Sep 14;477(7365):471-6. doi: 10.1038/nature10403.
5
Precise manipulation of chromosomes in vivo enables genome-wide codon replacement.精确地在体内操纵染色体可实现全基因组密码子替换。
Science. 2011 Jul 15;333(6040):348-53. doi: 10.1126/science.1205822.
6
Creation of a bacterial cell controlled by a chemically synthesized genome.人工合成基因组控制的细菌细胞的创建。
Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20.
7
Programming cells by multiplex genome engineering and accelerated evolution.通过多重基因组工程和加速进化对细胞进行编程。
Nature. 2009 Aug 13;460(7257):894-898. doi: 10.1038/nature08187. Epub 2009 Jul 26.
8
Enzymatic assembly of DNA molecules up to several hundred kilobases.长达数百千碱基的DNA分子的酶促组装。
Nat Methods. 2009 May;6(5):343-5. doi: 10.1038/nmeth.1318. Epub 2009 Apr 12.
9
One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.在酵母中一步组装25个重叠DNA片段以形成完整的合成生殖支原体基因组。
Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20404-9. doi: 10.1073/pnas.0811011106. Epub 2008 Dec 10.
10
Teaching synthetic biology, bioinformatics and engineering to undergraduates: the interdisciplinary Build-a-Genome course.向本科生教授合成生物学、生物信息学和工程学：跨学科的构建基因组课程。
Genetics. 2009 Jan;181(1):13-21. doi: 10.1534/genetics.108.096784. Epub 2008 Nov 17.
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