Preuß Annegret, Saltsman Irena, Mahammed Atif, Pfitzner Michael, Goldberg Israel, Gross Zeev, Röder Beate
Department of Physics, Humboldt - Universität zu Berlin, Newtonstraße 15, 12489 Berlin, Germany.
Schulich Faculty of Chemistry, Technion - Israel Institute of Technology, Haifa 32000, Israel.
J Photochem Photobiol B. 2014 Apr 5;133:39-46. doi: 10.1016/j.jphotobiol.2014.02.013. Epub 2014 Mar 4.
The photodynamic effect, originally used in photodynamic therapy (PDT) for the treatment of different diseases, e.g. of cancer, has recently been introduced for the inactivation of bacteria. Mold fungi, which provoke health problems like allergies and diseases of the respiratory tract, are even more resistant and their biology is also very different. This study presents the development of four new photosensitizers, which, in combination with low doses of white light, inhibit the germination of mold fungi spores. Two of them even cause lethal damage to the conidia (spores) which are responsible for the spreading of mold fungi. The photoactivity of the newly synthesized corroles was obtained by their application on three different mold fungi: Aspergillus niger, Cladosporium cladosporoides, and Penicillium purpurgenum. To distinguish between inactivation of germination and permanent damage, the fungi were first incubated under illumination for examination of photosensitizer-induced growth inhibition and then left in darkness to test the survival of the conidia. None of the compounds displayed dark toxicity, but all of them attenuated or prevented germination when exposed to light, and the positively charged complexes induced a complete damage of the conidia.
光动力效应最初用于光动力疗法（PDT）治疗不同疾病，如癌症，最近已被用于细菌的灭活。引发过敏和呼吸道疾病等健康问题的霉菌更具抗性，其生物学特性也非常不同。本研究展示了四种新型光敏剂的研发，这些光敏剂与低剂量白光结合可抑制霉菌孢子的萌发。其中两种甚至会对负责霉菌传播的分生孢子（孢子）造成致命损伤。通过将新合成的卟吩应用于三种不同的霉菌：黑曲霉、枝孢菌和紫青霉，获得了其光活性。为区分萌发失活和永久性损伤，先将真菌在光照下培养以检测光敏剂诱导的生长抑制，然后置于黑暗中测试分生孢子的存活率。所有化合物在黑暗中均无毒性，但在光照下均能减弱或阻止萌发，且带正电荷的络合物会导致分生孢子完全损伤。
