Doğan Murat, Çağlı Sedat, Yüce İmdat, Bayram Ali, Somdaş Mehmet Akif, Karataş Duran, Cihan Mehmet Celalettin, Yüksel Fatih, Güney Ercihan
Research and Traınıng Hospital, Kayseri, Turkey,
Eur Arch Otorhinolaryngol. 2015 Mar;272(3):689-94. doi: 10.1007/s00405-014-3009-3. Epub 2014 Mar 28.
In this study, the correlation between neck metastasis and recurrence was investigated by studying specimens of tongue squamous cell carcinoma patients immunohistochemical with survivin antibodies in the primary biopsy. A retrospective review was conducted at the Academic University Hospital. 46 patients who had squamous cell carcinoma of the tongue, who underwent various types of glossectomy and neck dissections between 1991 and 2008, were evaluated. The patient's sex, TNM staging, differentiation and recurrence rates were analyzed. There were 20 T1 patients and 26 T2 patients; 27 of the patients were N0 and 19 had metastatic lymph nodes in the neck. Survivin antibodies were applied with streptavidin-biotin method to the sections that were prepared from the primary tumor biopsy specimens of the patients. The correlation between neck metastasis and recurrence and survivins' immunohistochemical staining was analyzed with statistical methods. There were no significant differences between the patient's age, sex, tumor's T stage, tumor differentiation and survivin staining density. Survivin staining was positive in 15 (79 %) of 19 patients with neck metastasis, while it was positive in 16 (59 %) of 27 patients without neck metastasis. Eleven (79 %) of the 14 patients who had recurrence and all 6 patients who had neck recurrence only were stained by survivin. Expression of nuclear and cytoplasmic survivin can be a useful marker for predicting cervical lymph node metastasis in T1-T2 tumors in tongue SCC.
在本研究中，通过对舌鳞状细胞癌患者原发活检标本进行survivin抗体免疫组化研究，探讨颈部转移与复发之间的相关性。在学术大学医院进行了一项回顾性研究。对1991年至2008年间接受各种类型舌切除术和颈部清扫术的46例舌鳞状细胞癌患者进行了评估。分析了患者的性别、TNM分期、分化程度和复发率。其中T1期患者20例，T2期患者26例；27例患者为N0，19例患者颈部有转移性淋巴结。采用链霉亲和素-生物素法将survivin抗体应用于患者原发肿瘤活检标本制备的切片上。用统计学方法分析颈部转移与复发以及survivin免疫组化染色之间的相关性。患者的年龄、性别、肿瘤的T分期、肿瘤分化程度和survivin染色密度之间无显著差异。19例颈部转移患者中有15例（79%）survivin染色呈阳性，而27例无颈部转移患者中有16例（59%）呈阳性。14例复发患者中有11例（79%）以及仅颈部复发的所有6例患者survivin染色均呈阳性。核型和胞质型survivin的表达可能是预测舌鳞状细胞癌T1-T2期肿瘤颈部淋巴结转移的有用标志物。
