Durand D, Saint-Cyr J A, Gurevich N, Carlen P L
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106.
Brain Res. 1989 Jan 16;477(1-2):373-7. doi: 10.1016/0006-8993(89)91430-3.
The effects of chronic ethanol intake were studied on the morphology of rat hippocampal granule cells. Sprague-Dawley rats were exposed to ethanol in a liquid diet for 5 months followed by a 3 week withdrawal period. A control group was fed similar amounts of the same diet but with ethanol replaced by maltose-dextrins. Intracellular recordings were performed using the hippocampal slice preparation and the granule cells from animals of both groups were injected with HRP. The ethanol treatment produced a significant increase in the average length of the dendrites of granule cells compared to the control group. Chronic ethanol intake produced a decrease in the number of dendrites in the proximal region of the tree (80-180 microns) but also a significant increase in the number of dendrites in the distal portion (260-340 microns). The decrease in the number of proximal dendrites suggests that ethanol could be affecting a population of neurons with afferent inputs in the proximal region of the tree or that ethanol could interfere with the normal maturation processes of the granule cells. The increase in the number of dendrites in the distal region of the tree suggests, however, an accelerated growth or sprouting of dendrites in the molecular layer.
研究了长期摄入乙醇对大鼠海马颗粒细胞形态的影响。将斯普拉格-道利大鼠置于含乙醇的液体饲料中5个月，随后有3周的戒断期。对照组给予等量的相同饲料，但用麦芽糖糊精替代乙醇。使用海马脑片制备进行细胞内记录，并向两组动物的颗粒细胞中注射辣根过氧化物酶（HRP）。与对照组相比，乙醇处理使颗粒细胞树突的平均长度显著增加。长期摄入乙醇使树突近端区域（80 - 180微米）的树突数量减少，但也使远端部分（260 - 340微米）的树突数量显著增加。近端树突数量的减少表明乙醇可能影响了树突近端区域有传入输入的神经元群体，或者乙醇可能干扰了颗粒细胞的正常成熟过程。然而，树突远端区域树突数量的增加表明分子层中树突的生长或发芽加速。
