Hermann E, Fleischer B, Mayet W J, Poralla T, Meyer zum Büschenfelde K H
I. Medizinische Klinik und Poliklinik, Johannes Gutenberg Universität, Mainz, FRG.
Clin Exp Immunol. 1989 Mar;75(3):365-70.
From synovial fluids of two patients with reactive arthritis following Yersinia enterocolitica infection, T lymphocyte clones were obtained that showed proliferative responses to Y. enterocolitica. The responses required autologous T-cell-depleted peripheral blood mononuclear cells as antigen presenting cells. Three clones were studied in detail; two of them showed a marked and specific response to Yersinia antigens alone, the other one recognized both Yersinia and Salmonella typhimurium antigens. The antigen-specific proliferation of the clones could be completely blocked by monoclonal antibodies to HLA-DR. These experiments show that synovial T lymphocytes presumably involved in the in situ immune response to microbial antigens triggering reactive arthritis can be cloned directly from the site of inflammation.
从两名小肠结肠炎耶尔森菌感染后反应性关节炎患者的滑液中，获得了对小肠结肠炎耶尔森菌有增殖反应的T淋巴细胞克隆。这些反应需要自体T细胞耗竭的外周血单核细胞作为抗原呈递细胞。对三个克隆进行了详细研究；其中两个克隆仅对耶尔森菌抗原有显著的特异性反应，另一个克隆则识别耶尔森菌和鼠伤寒沙门氏菌抗原。克隆的抗原特异性增殖可被抗HLA-DR单克隆抗体完全阻断。这些实验表明，可能参与对引发反应性关节炎的微生物抗原的原位免疫反应的滑膜T淋巴细胞可直接从炎症部位克隆获得。
