Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, Turkey.
Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, Turkey.
Food Chem. 2014 Aug 15;157:323-31. doi: 10.1016/j.foodchem.2014.02.053. Epub 2014 Feb 22.
A microwave-assisted extraction (MAE) process for polyphenols from three wild edible mushrooms was studied. The optimal extraction conditions were found to be methanol concentration of 80%, extraction temperature of 80 °C, and extraction time of 5 min. Different antioxidant assays (i.e., total antioxidant capacity (TAC) and total phenolic content (TPC)) were utilized to evaluate the antioxidant capacity of the methanolic extracts of Terfezia boudieri Chatin, Boletus edulis, and Lactarius volemus. The reactive species scavenging activities of these extracts were also investigated in vitro. High contents of phenolic and flavonoid compounds may be the major contributors to the observed high antioxidant activities of these extracts. B. edulis showed the higher TAC and TPC; highest inhibitory effect on DPPH and on other studied reactive oxygen species (ROS). MAE showed obvious advantages of high extraction efficiency with lower solvent consumption in terms of high antioxidant capacity/activity of extracts achieved within the shortest time.
研究了一种从三种野生食用蘑菇中提取多酚的微波辅助提取(MAE)工艺。发现最佳提取条件为甲醇浓度 80%、提取温度 80°C 和提取时间 5 分钟。利用不同的抗氧化测定方法(即总抗氧化能力(TAC)和总酚含量(TPC))来评估 Terfezia boudieri Chatin、Boletus edulis 和 Lactarius volemus 甲醇提取物的抗氧化能力。还在体外研究了这些提取物对活性物质的清除活性。高含量的酚类和类黄酮化合物可能是这些提取物表现出高抗氧化活性的主要原因。B. edulis 表现出更高的 TAC 和 TPC;对 DPPH 和其他研究的活性氧(ROS)的抑制作用最强。MAE 具有明显的优势,即在最短的时间内以较低的溶剂消耗实现高提取效率和提取物的高抗氧化能力/活性。