Fredotović Željana, Šprung Matilda, Soldo Barbara, Ljubenkov Ivica, Budić-Leto Irena, Bilušić Tea, Čikeš-Čulić Vedrana, Puizina Jasna
Department of Biology, Faculty of Science, University of Split, R. Boškovića 33, 21000 Split, Croatia.
Department of Chemistry, Faculty of Science, University of Split, R. Boškovića 33, 21000 Split, Croatia.
Molecules. 2017 Mar 11;22(3):448. doi: 10.3390/molecules22030448.
Here, we report a comparative study of the phytochemical profile and the biological activity of two onion extracts, namely L. and × (Clementi ex Visiani 1842), members of the family Amaryllidaceae. The identification of flavonoids and anthocyanins, and their individual quantities, was determined by high-performance liquid chromatography (HPLC). The potency of both extracts to scavenge free radicals was determined by the DPPH (2,2'-diphenyl-1-picrylhydrazyl) radical-scavenging activity and oxygen radical absorbance capacity (ORAC) methods. The DNA protective role was further tested by the single-cell gel electrophoresis (COMET) assay and by Fenton's reagent causing double-strand breaks on the closed circular high copy pUC19 plasmid isolated from . In the presence of both extracts, a significant decrease in DNA damage was observed, which indicates a protective role of and × on DNA strand breaks. Additionally, cytotoxicity was tested on glioblastoma and breast cancer cell lines. The results showed that both extracts had antiproliferative effects, but the most prominent decrease in cellular growth was observed in glioblastoma cells.
在此，我们报告了对两种葱属提取物（即石蒜科植物L.和×（Clementi ex Visiani 1842））的植物化学特征和生物活性的比较研究。通过高效液相色谱法（HPLC）对黄酮类化合物和花青素进行鉴定，并测定其各自的含量。通过DPPH（2,2'-二苯基-1-苦基肼）自由基清除活性和氧自由基吸收能力（ORAC）方法测定两种提取物清除自由基的能力。通过单细胞凝胶电泳（彗星）试验以及使用从分离出的闭合环状高拷贝pUC19质粒上引起双链断裂的芬顿试剂，进一步测试了DNA保护作用。在两种提取物存在的情况下，观察到DNA损伤显著减少，这表明L.和×对DNA链断裂具有保护作用。此外，还对胶质母细胞瘤和乳腺癌细胞系进行了细胞毒性测试。结果表明，两种提取物均具有抗增殖作用，但在胶质母细胞瘤细胞中观察到细胞生长最显著的下降。
