Charles University, Institute of Geochemistry, Mineralogy and Mineral Resources, Prague, Czech Republic.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Apr;106:99-103. doi: 10.1016/j.saa.2012.12.081. Epub 2013 Jan 8.
Laboratory cultures of a number of red extremely halophilic Archaea (Halobacterium salinarum strains NRC-1 and R1, Halorubrum sodomense, Haloarcula valismortis) and of Salinibacter ruber, a red extremely halophilic member of the Bacteria, have been investigated by Raman spectroscopy using 514.5nm excitation to characterize their carotenoids. The 50-carbon carotenoid α-bacterioruberin was detected as the major carotenoid in all archaeal strains. Raman spectroscopy also detected bacterioruberin as the main pigment in a red pellet of cells collected from a saltern crystallizer pond. Salinibacter contains the C40-carotenoid acyl glycoside salinixanthin (all-E, 2'S)-2'-hydroxy-1'-[6-O-(methyltetradecanoyl)-β-d-glycopyranosyloxy]-3',4'-didehydro-1',2'-dihydro-β,ψ-carotene-4-one), for which the Raman bands assignments of are given here for the first time.
已使用 514.5nm 激发的拉曼光谱法对一些红色极端嗜盐古菌（盐杆菌 NRC-1 和 R1 菌株、盐沼盐球菌、耐盐盐球菌）和细菌的红色极端嗜盐成员盐红杆菌的实验室培养物进行了研究，以表征它们的类胡萝卜素。在所有古菌菌株中均检测到 50 碳类胡萝卜素 α-菌红菌素作为主要类胡萝卜素。拉曼光谱法还检测到，从盐田结晶池的红色颗粒细胞中收集的红色颗粒中，菌红菌素是主要色素。盐杆菌含有 C40-类胡萝卜素酰基糖苷盐黄素（全-E，2'S）-2'-羟基-1'-[6-O-（甲基十四烷酰基）-β-d-吡喃葡萄糖氧基]-3'，4'-二脱氢-1'，2'-二氢-β，ψ-类胡萝卜素-4-酮），本文首次给出了其拉曼带的分配。
