Ohtsuki Y, Takahashi K, Sonobe H, Iwata J, Hayashi K
Department of Pathology, Kochi Medical School, Nankoku, Japan.
Acta Pathol Jpn. 1988 Sep;38(9):1167-73. doi: 10.1111/j.1440-1827.1988.tb02389.x.
S-100 beta-positive T-lymphocytes in human peripheral blood were shown to be definitely T-cells containing the S-100 beta subunit in their cytoplasm by double immunostaining using immunoferritin labeling for CD8 antigen and immunoperoxidase labeling for cytoplasmic S-100 beta subunit at the ultrastructural level. These results ultrastructurally confirmed the presence of double markers of S-100 beta-positive T-lymphocytes seen by light microscopic double immunostaining. Furthermore, this double immunostaining method for electron microscopy is applicable to other kinds of cells for the simultaneous detection of two different antigens present both on the cell surface and in the cytoplasm.
通过免疫铁蛋白标记CD8抗原和免疫过氧化物酶标记细胞质S - 100β亚基的双重免疫染色，在超微结构水平上证实人类外周血中S - 100β阳性T淋巴细胞确实是细胞质中含有S - 100β亚基的T细胞。这些结果在超微结构上证实了光镜双重免疫染色所见的S - 100β阳性T淋巴细胞双重标记物的存在。此外，这种用于电子显微镜的双重免疫染色方法适用于其他类型的细胞，可同时检测存在于细胞表面和细胞质中的两种不同抗原。
