多种中草药及一种含草药牙膏对与口臭相关的挥发性硫化物的影响：一项体外分析。

Effect of a variety of Chinese herbs and an herb-containing dentifrice on volatile sulfur compounds associated with halitosis: An in vitro analysis.

作者信息

Li Ming-Yu, Wang Jun, Xu Zhu-Ting

机构信息

Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, 9th People's Hospital, Medical College, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Shanghai 8th Hospital, Shanghai, People's Republic of China.

出版信息

Curr Ther Res Clin Exp. 2010 Apr;71(2):129-40. doi: 10.1016/j.curtheres.2010.03.002.

DOI:10.1016/j.curtheres.2010.03.002

PMID:24683259

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3967350/

Abstract

BACKGROUND

The principal components of halitosis are volatile sulfur compounds (VSCs) such as hydrogen sulfide, methyl mercaptan, and dimethylsulfide or compounds such as butyric acid, propionic acid, putrescine, and cadaverine.

OBJECTIVE

The aim of this study was to evaluate the effect of Chinese herbs on VSCs in vitro.

METHODS

Saliva samples from volunteers were used as the source for the evaluation of bacterial activity and VSC inhibition. Extracted substances from Chinese herbs were identified by VSC inhibition tests with a Halimeter and microbial sensitivity testing. The effectiveness on halitosis was compared between a dentifrice containing one of the effective Chinese herbs (ie, chrysanthemum flower [Chrysanthemum morifolium flos]), 4 commercially available antihalitosis dentifrices, and a positive control that received no treatment.

RESULTS

Ten volunteers provided saliva samples for VSC testing. Of the 40 herbs tested, 14 extracts had percent inhibition rates of VSCs >50%. Ten herbs showed greatest effect against all culturable microorganisms with bacterial inhibition >70%. There was a weak positive correlation between bacteriostasis and the anti-VSC activity of the herbs with a correlation coefficient of 0.2579 (Pearson). The mean (SD) values of the VSC testing were as follows: dentifrice containing chrysanthemum flower, 55.91 (8.16) ppb; Crest Tea Refreshing Dentifrice®, 48.39 (7.48) ppb (P = NS); Cortex Phellodendri Dentifrice®, 139.90 (14.70) ppb (P < 0.01); Colgate Total Plus Whitening®, 120.94 (15.58) ppb (P < 0.01); Zhong Hua Chinese Herbs Dentifrice®, 136.96 (13.06) ppb (P < 0.01); and positive control, 312.38 (28.58) ppb (P < 0.01).

CONCLUSIONS

Of 40 herbs tested, 14 Chinese herbs were found to be effective for VSC inhibition. A dentifrice containing chrysanthemum flower reduced the formation of VSC in vitro, showing a significantly greater effect than the control group and 3 of 4 dentifrices already on the market.

摘要

背景

口臭的主要成分是挥发性硫化物（VSCs），如硫化氢、甲硫醇和二甲基硫醚，或丁酸、丙酸、腐胺和尸胺等化合物。

目的

本研究旨在评估中药在体外对挥发性硫化物的影响。

方法

使用志愿者的唾液样本作为评估细菌活性和抑制挥发性硫化物的来源。通过使用Halimeter进行挥发性硫化物抑制试验和微生物敏感性测试来鉴定中药提取物。比较含有一种有效中药（即菊花[Chrysanthemum morifolium flos]）的牙膏、4种市售抗口臭牙膏和未接受治疗的阳性对照对口臭的疗效。

结果

10名志愿者提供了用于挥发性硫化物测试的唾液样本。在测试的40种草药中，14种提取物对挥发性硫化物的抑制率>50%。10种草药对所有可培养微生物显示出最大效果，细菌抑制率>70%。草药的抑菌作用与抗挥发性硫化物活性之间存在弱正相关，相关系数为0.2579（Pearson）。挥发性硫化物测试的平均值（标准差）如下：含菊花的牙膏，55.91（8.16）ppb；佳洁士茶爽牙膏，48.39（7.48）ppb（P = 无显著性差异）；黄柏牙膏，139.90（14.70）ppb（P < 0.01）；高露洁全效美白牙膏，120.94（15.58）ppb（P < 0.01）；中华中草药牙膏，136.96（13.06）ppb（P < 0.01）；阳性对照，312.38（28.58）ppb（P < 0.01）。