Jiang Y, Yao L, Li F, Tan Z, Zhai Y, Wang L
Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Lett Appl Microbiol. 2014 Aug;59(2):147-54. doi: 10.1111/lam.12258. Epub 2014 Apr 19.
This study was aimed to evaluate the antimicrobial resistance and molecular resistance mechanisms of 87 Vibrio parahaemolyticus isolates from cultured sea cucumbers (Apostichopus japonicus). The results showed that all isolates were resistant to ampicillin and cephazolin, fewer of them were resistant to streptomycin (43·7%), cefuroxime sodium (18·4%), tetracycline (4·6%), sulphamethoxazole/trimethoprim (2·3%) and four quinolones (2·3%). More than half (56·2%) of the isolates displayed multiple resistance to at least three antimicrobials. The resistance genes were detected in all antimicrobial-resistant isolates except two tetracycline-resistant isolates. Among all these tested resistance genes, blaTEM , sul2, strA and strB were predominant, and none of blaSHV , blaCTX-M , blaOXA , sul1, sul3, tetA, tetM and tetQ genes was detected. Point mutations were found in quinolone resistance-determining regions of gyrA and parC genes in quinolone-resistant isolates. All isolates harboured class 1 integrons but only one carried gene cassette without any resistance genes, and none of them was positive to class 2, 3 integrons and SXT constins. These results indicate that the antimicrobial-resistant V. parahaemolyticus isolates from sea cucumbers and resistance genes could be potential risks to public health or other environments.
This study is the first report on characterization of antimicrobial resistance of Vibrio parahaemolyticus from sea cucumbers (Apostichopus japonicus). Our findings reveal a high level of resistance to some antimicrobials and prevalence of the resistance genes in V. parahaemolyticus isolates from sea cucumbers and underline the need for prudent use of antimicrobials in aquaculture to minimize the spread of antimicrobial-resistant V. parahaemolyticus.
本研究旨在评估87株从养殖海参（刺参）中分离出的副溶血性弧菌的抗菌药物耐药性及分子耐药机制。结果显示，所有分离株均对氨苄西林和头孢唑林耐药，较少分离株对链霉素（43.7%）、头孢呋辛钠（18.4%）、四环素（4.6%）、磺胺甲恶唑/甲氧苄啶（2.3%）和四种喹诺酮类药物（2.3%）耐药。超过半数（56.2%）的分离株对至少三种抗菌药物表现出多重耐药。除两株四环素耐药分离株外，在所有抗菌药物耐药分离株中均检测到耐药基因。在所有这些检测的耐药基因中，blaTEM、sul2、strA和strB占主导地位，未检测到blaSHV、blaCTX-M blaOXA、sul1、sul3、tetA、tetM和tetQ基因。在喹诺酮耐药分离株的gyrA和parC基因的喹诺酮耐药决定区发现了点突变。所有分离株均携带1类整合子，但只有一株携带无任何耐药基因的基因盒，且均未对2类、3类整合子和SXT 元件呈阳性。这些结果表明，从海参中分离出的抗菌药物耐药副溶血性弧菌及其耐药基因可能对公众健康或其他环境构成潜在风险。
本研究是关于海参（刺参）中副溶血性弧菌抗菌药物耐药性特征的首次报道。我们的研究结果揭示了海参中副溶血性弧菌对某些抗菌药物的高度耐药性以及耐药基因的流行情况，并强调了在水产养殖中谨慎使用抗菌药物以尽量减少抗菌药物耐药副溶血性弧菌传播的必要性。
