Dong Fulu, Zhang Yuan, Xia Fei, Yang Yi, Xiong Sidong, Jin Liping, Zhang Jinping
Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China.
Institutes of Biology and Medical SciencesSoochow University, Suzhou, Jiangsu Province 215123, People's Republic of ChinaLaboratory for Reproductive ImmunologyHospital and Institute of Obstetrics and Gynecology, Fudan University Shanghai Medical College, No. 413, Zhaozhou Road, Shanghai 200011, People's Republic of China
Reproduction. 2014 Jul;148(1):33-41. doi: 10.1530/REP-14-0095. Epub 2014 Mar 31.
MicroRNAs (miRNAs) are non-coding RNA molecules of about 22 nucleotides that involved in post-transcriptional gene regulation. Evidence indicates that miRNAs play essential roles in endometriosis, pre-eclampsia, infertility and other reproductive system diseases. However, whether miRNAs are involved in recurrent spontaneous abortion (RSA) is unclear. In this work, we analysed the miRNA expression profiles in six pairs of villus or decidua from RSA patients and normal pregnancy (NP) women using a human miRNA microarray. Some of the chip results were confirmed by RT-qPCR. In the villi of RSA patients, expression of hsa-miR-184, hsa-miR-187 and hsa-miR-125b-2 was significantly higher, while expression of hsa-miR-520f, hsa-miR-3175 and hsa-miR-4672 was significantly lower, comparing with those of NP control. As well, a total of five miRNAs (hsa-miR-517c, hsa-miR-519a-1, hsa-miR-522, hsa-miR-520h and hsa-miR-184) were upregulated in the decidua of RSA patients. The target genes of these differentially expressed miRNAs were predicted by miRWalk, and we speculate a network of miRNA regulating RSA by target genes function on adhesion, apoptosis and angiogenesis. Our study may help clarify the molecular mechanisms which are involved in the progression of RSA, and provide a reference for future research.
微小RNA（miRNA）是一类约22个核苷酸的非编码RNA分子，参与转录后基因调控。有证据表明，miRNA在子宫内膜异位症、先兆子痫、不孕症及其他生殖系统疾病中发挥着重要作用。然而，miRNA是否参与复发性自然流产（RSA）尚不清楚。在本研究中，我们使用人类miRNA芯片分析了6对RSA患者和正常妊娠（NP）妇女的绒毛或蜕膜组织中的miRNA表达谱。部分芯片结果通过RT-qPCR进行了验证。与NP对照组相比，RSA患者绒毛中hsa-miR-184、hsa-miR-187和hsa-miR-125b-2的表达显著升高，而hsa-miR-520f、hsa-miR-3175和hsa-miR-4672的表达显著降低。此外，RSA患者蜕膜中共有5种miRNA（hsa-miR-517c、hsa-miR-519a-1、hsa-miR-522、hsa-miR-520h和hsa-miR-184）表达上调。通过miRWalk预测了这些差异表达miRNA的靶基因，我们推测miRNA通过靶基因在黏附、凋亡和血管生成方面的功能调控RSA的网络。我们的研究可能有助于阐明RSA发生发展的分子机制，并为未来研究提供参考。
